Destaining the hematoxylin (with acid alcohol?) won't alter the DAB
staining.  DAB staining is extremely stable, and difficult to remove
chemically even when you want to.

> ----------
> From: 	histonet-bounces@lists.utsouthwestern.edu on behalf of
> cacartwr@mdanderson.org
> Sent: 	Tuesday, June 27, 2006 5:23 AM
> To: 	histonet@lists.utsouthwestern.edu
> Subject: 	[Histonet] destaining and DAB
> 
> I have some slides that I did IHC on with DAB and then I counterstained 
> with Hematoxylin.  I want to destain the hematoxylin and re-do it.  Will 
> destaining affect the DAB?
> Thanks,
> Carrie
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> 

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