[Histonet] to perfuse or not to perfuse...
I hate to broach such a seemingly banal question, but I searched the
archives and couldn't find exactly what I need. So here goes...
I have been using a virus to express EGFP in mice brains with great
results. I perfuse the mice, post-fix overnight, immerse in 25%
sucrose overnight, section at 40 microns, mount and visualize the
native fluorescence. Now I want to send my virus to another lab to
test, however, they don't know how to perfuse. Do you think the
native fluorescence will be retained if the brain is collected fresh
and immersed in NBF?
Are there any suggestions for preparing the brain other than by
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