[Histonet] Re:Gr-1 staining in mouse spleen (Herber, Donna L.)
I've had great results in mouse granulocytic tumors
with Gr-1 but my protocol was very standard you can check out the protocol
in this journal article.
Kyriaki Dunussi-Joannopoulos, Jamie Erickson et al... Efficacious
immunomodulatory activity of the chemokine stromal cell-derived factor 1
(SDF-1): local secretion of SDF-1 at the tumor site serves as T-cell
chemoattractant and mediates T-cell-dependent antitumor responses. Blood
100(5): 1551-1558, 2002 Sept.
I used frozen sections and the Gr-1 antibody from BD @ 2ug/ml (LY-6G),
RB6-8C5 Catalog #: 550291 (FOR IHC) we used this to check that
granulocytes in and around that tumor.
I think you may be using the wrong antibody this one is stated to work in
IHC by BD( CAT # 550291,RB68C5) and is the same clone I use. You might
want to look into that.
Sr. Research Associate
Abbott Bioresearch Center
100 Research Drive
Worcester, MA 01605-4341
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