HI Donna,
                     I've had great results in mouse granulocytic  tumors 
with Gr-1 but my protocol was very standard you can check out the protocol 
in this journal article.
 
Kyriaki Dunussi-Joannopoulos, Jamie Erickson et al... Efficacious 
immunomodulatory activity of the chemokine stromal cell-derived factor 1 
(SDF-1): local secretion of SDF-1 at the tumor site serves as T-cell 
chemoattractant and mediates T-cell-dependent antitumor responses. Blood 
100(5): 1551-1558, 2002 Sept.

I used frozen sections and the Gr-1 antibody from BD @ 2ug/ml (LY-6G), 
RB6-8C5 Catalog #: 550291 (FOR IHC) we used this to check that 
granulocytes in and around that tumor. 

I think you may be using the wrong antibody this one is stated to work in 
IHC by BD( CAT # 550291,RB68C5) and is the same clone I use.  You might 
want to look into that.

Jamie
_______________________________
Jamie Erickson
Sr. Research Associate 
Department: DSMP
Abbott Bioresearch Center
100 Research Drive
Worcester, MA 01605-4341
508-688-3134
FAX: 508-793-4895
e-mail: jamie.erickson@abbott.com
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