Re: [Histonet] section adhesion on slides

From:Geoff McAuliffe

Hi Olivier:

    Coat your own slides. Here is the method I use.

  "Silane" or APES coating

  <>      Excellent for sections that will be subjected to trypsin 
digestion or other harsh treatments (saponification). Add 2 ml of 
3-aminopropyltriethoxysilane (Sigma A-3648) to 98 ml of acetone. Dip 
clean slides in this mixture for 5 seconds, drain and rinse in two 
changes of acetone, 1 minute each. After about 35-40 slides discard the 
first acetone, move the second rinse up to be the first rinse and make a 
fresh second rinse. Air dry the slides in a vertical position and store 
in a dust free box. Keeps indefinitely.  <>

Rentrop, M., B. Knapp, H. Winter and J. Schweizer. 
Aminoalkylsilane-treated slides as support for in situ hybridization of 
keratin cDNA's to frozen tissue sections under varying fixation and 
pretreatment conditions. Histochemical J. 18:271-276, 1986.

Note that clean is in bold type. I have never trusted "precleaned" 
slides, probably because I am old enough to remember when precleaned 
meant the absence of large pieces of dirt and not much else. I always 
wash my precleaned slides in hot, soapy water, rinse thoroughly, rinse 
in distilled water, dry in a clean (not paraffin) oven. Then coat with 
silane, albumin, gelatin, poly-L-lysine, etc.


Olivier Leroux wrote:

>Currently we are using vectabond coating to treat our slides for a good adhesion
>of technovit 7100 sections. Does anybody know a cheaper alternative?
>Olivier Leroux
>Ghent University
>Department of Biology - Pteridological Section
>K.L. Ledeganckstraat 35
>B-9000 Belgium
>Histonet mailing list

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

Histonet mailing list

<< Previous Message | Next Message >>