Re: [Histonet] brain tissue sections

From:"Nancy Lemke"

Hi Patsy,
The problem you describe is because the sections have not dried enough.  LuAnn is exactly correct about air-drying over night.  If you don't air-dry, the sections can look like they have exploded off of the slide as soon as you hit the xylene.  This tip was given to me many eons ago by a technical specialist at Miles in Elkhart, Ind.  He worked with  VIP processors and had encountered this problem with one of his customers.  After trying everything, they accidentally left the sections in a rack overnight and the problem was gone!  I have blessed him many times over the years for saving the remaining shreds of my sanity.  It does not matter if you use plain glass, gel-coated or charged slides.  Rodent brains need the extra room temperature drying and I even do the same with human samples because I believe in superstitious behavior!
Nancy Lemke
Research Coordinator
Hermelin Brain Tumor Center
Henry Ford Hospital
-----Original message-----
From: "Patsy Ruegg"
Date: Thu, 08 Jun 2006 14:04:26 -0400
Subject: [Histonet] brain tissue sections

> We are having a terrible time with our ffpe 4 micron rat brain tissue
> sections falling off the slides, we are using silane coated slides, air
> drying on edge on a small fan, then heating on a slide warmer flat at 55dc
> for 1 hour to overnight.  Please help.  The sections are falling off during
> deparaffinizing thru xylene and alcohols, haven't even gotten to HIER and
> IHC yet.
> thanks for your help,
> Patsy
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech, LLC
> Fitzsimmons BioScience Park
> 12635 Montview Blvd. Suite 215
> Aurora, CO 80010
> P-720-859-4060
> F-720-859-4110
> wk email
> web site  
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