Re: [Histonet] Minimizing shrinkage
Hi Tim:
Any processing will induce some shrinkage so my first suggestion is
to measure fresh or fixed tissue with a dissecting microscope. If that
is not possible I suggest a thorough fixation in formalin or
formalin+glutaraldehyde followed by embedding in an epoxy resin (Epon
substitute). Much less shrinkage in epoxies as compared to wax or
methacrylates.
Geoff
Timothy Macatee wrote:
>I have someone who wants to measure cardiac vessel diameter. Does anyone
>have an idea of the best process to avoid shrinkage of the tissue? Notice,
>how I made no reference to any situation comedy television shows.
>
>Tim
>
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
**********************************************
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<< Previous Message | Next Message >>