Re: [Histonet] A Call for Help from Melbourne/OZ

From:Geoff McAuliffe

THE person for zinc is G. Danscher, do a PubMed search.for his name or 
"detection of zinc".


Bruce Abaloz wrote:

> Dear Histonetters,
> My name is Scott Sinclair, and I am a PhD student in the Department of 
> Genetics (@ The University of Melbourne/Australia) and am hoping to 
> get your advice on the following:
> I have been using a fluorescent dye (Zinpyr) to try to image Zinc in 
> the roots of Arabidopsis seedlings/plant, using a confocal microscope. 
> I'm also interested in looking at paraffin sections of roots, 
> therefore wondering if there is a 'fixing' process that will leave the 
> intracellular localisation of Zinc undisturbed while examining 
> sections/Will routine fixation in 10% NBF or 70% ETOH be fine? I know 
> you have to fix sections before looking at protein or DNA/RNA, and was 
> wondering if it can be done for ions also?
> Regards,
> Scott Sinclair (sent through Bruce)

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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