RE: More on Re: [Histonet] RE: DAB disposal[Scanned]

From:"Emma JONES"

Absolutely agree with you. The less waste thrown down the sink the better. 

You will notice that Joe said he used the same method I mentioned for his Dako waste. Maybe they recommended it, or perhaps like myself (excuse me, Joe for assuming here) it is a method he has heard of and used for a number of years, for me I knew of it a long time before joining, or even knowing about Ventana.

Various countries have a variety of methods of disposal, some can afford disposal companies, some cannot, some have guidelines set up for disposal, some do not. Within countries there is variation, and yes cost is an issue.
There are certainly kits that can be purchased for use with our instruments for disposal of waste, but that is up to the individual lab. Ultimately their preferred method of disposal is determined by their local guidelines.
I too would prefer to see more fixed guidelines, but with such lab/country variation will this be possible?

If you have any recommendations please share. 

Best regards

-----Original Message-----
From: Gayle Callis [] 
Sent: Tuesday, June 13, 2006 5:15 PM
To: Emma JONES;
Subject: More on Re: [Histonet] RE: DAB disposal[Scanned]

There has already been a great deal of discussion about this problem, and 
specific guidelines are often in place and implemented, even in remote 
areas and for small(or large) laboratories.  Have you done a Histonet 
Archive search on messages concerning this issue, it is there and been 
going on for a LONG time - well over 10 years or more - this is not a new 
issue.  Our laboratory has been well aware of DAB treatment for disposal 
and did it for a time, but opted NOT to do it long before we were required 
by regulation to cease but collect.    This treatment never addressed the 
by product issue i.e. what happens to the DAB after chemical 
treatment.  Are the byproducts going to be just as bad or worse?

If a laboratory can afford any kind of expensive immunostaining instrument 
such you sell, or even if they do manual IHC or other routine stains, then 
they should be able to afford collection/disposal services or seek such a 

We are a very small user of these compounds you mention i.e DAB, silver, 
any heavy metal compounds (chromic acid) even if only 25 mls of the stuff - 
it is collected for disposal.  Solvents never go down the drain.  As for 
bleaches, detergents, and disinfectants,  wastewater plants do allow 
certain things but these are also common to households, not just 

As far as I am concerned and there are a lot of others who might 
agree,  ALL labs, no matter if they are the "little guy" need to address 
and implement chemical waste collection and disposal.    The only time this 
could change is an exemption in a locale but laboratories need to find out 
if an exemption is in place before the dumping.  Once again, users beware - 
check out what is allowed for your area.

  I'm curious - you never said if this is your company's stand for DAB 
disposal or if it is just your comment.  Does Ventana advise/tell people to 
treat their DAB wastes for drain dumping?  It certainly would not be a good 
policy to do so and does not encourage people to be responsible for their 
chemical usage.

I liked the idea of one lady's  suggestion to use TBS DAB-Out, maybe that 
is an answer and I compliment her on her seeking a solution to the problem.

Gayle Callis HTL, HT, MT(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717

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