RE: [Histonet] why

From:"Bill Sinai"

One way to eliminate this problem is to put a drop of something like tween
in your formalin or whatever solution you store the cassttes in prior to
processing or the first solution on the processor. This reduces the problem
of surface tension of fluids across the small holes.

Bill Sinai 
Chief Hospital Scientist
Tissue Pathology ICPMR 
Westmead Hospital 
Westmead NSW 2145 
Phone 02 9845 7774 
Fax 02 9687 2330

-----Original Message-----
[] On Behalf Of Anthony F.
Sent: Wednesday, 14 June 2006 2:46 AM
Subject: RE: [Histonet] why

One thing that we found to help with the screened cassettes is to set the
cassette on a paper towel during grossing and adding formalin to the
cassette.  Let it flow thru to the towel before closing.  This seems to help
the fluids enter when we put them into the bucket.   And double check that
they do not float up.   You may have to give them a tap to displace that
bubble.   Since we have started this a year ago, we have only had a couple
of problems.

	-----Original Message----- 
	From: Joe Nocito [] 
	Sent: Mon 6/12/2006 7:45 PM 
	To: Santana, Diane; 
	Subject: Re: [Histonet] why

	do you the screened cassettes? I ran about 25 GI blocks this morning
	sat fixing since Saturday and I had to reprocess 2 of them. This
	every so often and it still puzzles me.
	    Sorry I don't have an answer, but you're not alone with this.
	    There I said it, ( in public too) I don't have all the answers.
	----- Original Message -----
	From: "Santana, Diane" 
	Sent: Monday, June 12, 2006 11:40 AM
	Subject: [Histonet] why
	> Hi
	> I am looking for some ideas to help with a problem I am still
having with
	> processing my GI bx's. What would cause my tissue to be raw? Not
all, not
	> some, only 1 or 2 pieces.
	> Example if I have a case with 4 bx's, maybe only 1 is raw, the
other 3 are
	> beautiful. All my solutions test at the right % and temperatures
are fine.
	> Question again, why does 1 piece of tissue come out raw, when the
	> pieces are fine?
	> thanks in advance,
	> Diane Santana
	> PMA
	> Haverhill, Mass.
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