RE: [Histonet] why
One way to eliminate this problem is to put a drop of something like tween
in your formalin or whatever solution you store the cassttes in prior to
processing or the first solution on the processor. This reduces the problem
of surface tension of fluids across the small holes.
Chief Hospital Scientist
Tissue Pathology ICPMR
Westmead NSW 2145
Phone 02 9845 7774
Fax 02 9687 2330
[mailto:firstname.lastname@example.org] On Behalf Of Anthony F.
Sent: Wednesday, 14 June 2006 2:46 AM
Subject: RE: [Histonet] why
One thing that we found to help with the screened cassettes is to set the
cassette on a paper towel during grossing and adding formalin to the
cassette. Let it flow thru to the towel before closing. This seems to help
the fluids enter when we put them into the bucket. And double check that
they do not float up. You may have to give them a tap to displace that
bubble. Since we have started this a year ago, we have only had a couple
From: Joe Nocito [mailto:email@example.com]
Sent: Mon 6/12/2006 7:45 PM
To: Santana, Diane; firstname.lastname@example.org
Subject: Re: [Histonet] why
do you the screened cassettes? I ran about 25 GI blocks this morning
sat fixing since Saturday and I had to reprocess 2 of them. This
every so often and it still puzzles me.
Sorry I don't have an answer, but you're not alone with this.
There I said it, ( in public too) I don't have all the answers.
----- Original Message -----
From: "Santana, Diane"
Sent: Monday, June 12, 2006 11:40 AM
Subject: [Histonet] why
> I am looking for some ideas to help with a problem I am still
> processing my GI bx's. What would cause my tissue to be raw? Not
> some, only 1 or 2 pieces.
> Example if I have a case with 4 bx's, maybe only 1 is raw, the
other 3 are
> beautiful. All my solutions test at the right % and temperatures
> Question again, why does 1 piece of tissue come out raw, when the
> pieces are fine?
> thanks in advance,
> Diane Santana
> Haverhill, Mass.
> Histonet mailing list
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