One thing that we found to help with the screened cassettes is to set the cassette on a paper towel during grossing and adding formalin to the cassette.  Let it flow thru to the towel before closing.  This seems to help the fluids enter when we put them into the bucket.   And double check that they do not float up.   You may have to give them a tap to displace that bubble.   Since we have started this a year ago, we have only had a couple of problems.
 
Tony

	-----Original Message----- 
	From: Joe Nocito [mailto:jnocito@satx.rr.com] 
	Sent: Mon 6/12/2006 7:45 PM 
	To: Santana, Diane; histonet@lists.utsouthwestern.edu 
	Cc: 
	Subject: Re: [Histonet] why
	
	

	Diane,
	do you the screened cassettes? I ran about 25 GI blocks this morning that
	sat fixing since Saturday and I had to reprocess 2 of them. This happens
	every so often and it still puzzles me.
	    Sorry I don't have an answer, but you're not alone with this.
	    There I said it, ( in public too) I don't have all the answers.
	
	Joe
	----- Original Message -----
	From: "Santana, Diane" 
	To: 
	Sent: Monday, June 12, 2006 11:40 AM
	Subject: [Histonet] why
	
	
	> Hi
	> I am looking for some ideas to help with a problem I am still having with
	> processing my GI bx's. What would cause my tissue to be raw? Not all, not
	> some, only 1 or 2 pieces.
	> Example if I have a case with 4 bx's, maybe only 1 is raw, the other 3 are
	> beautiful. All my solutions test at the right % and temperatures are fine.
	> Question again, why does 1 piece of tissue come out raw, when the other
	> pieces are fine?
	> thanks in advance,
	> Diane Santana
	> PMA
	> Haverhill, Mass.
	>
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