RE: [Histonet] overnight HIER

From:Gayle Callis


Linda is correct on this and Jules Elias has a publication on this in J of 
Histechnology, low heat antigen retrieval.  Access the publication via the 
NSH office at  I do not recall the buffer nor antigens he 
was retrieving, and not sure of the year of publication.

At 01:26 PM 6/6/2006, you wrote:
>In one of the workshops I attended in Toronto ( I wish I could remember
>the name of the presenter), they mentioned doing this but not at 60
>degrees C but a lower temp. like 37 or 45.  Hopefully this person will
>see your inquiry and respond.  I believe they did this for finicky
>antigens and tissues that were prone to come off the slides.
>Linda Sebree, HT(ASCP)
>University of Wisconsin Hospital & Clinics
>IHC/ISH Laboratory
>600 Highland Ave.
>Madison, WI 53792
>FAX: (608)262-7174
>-----Original Message-----
>[] On Behalf Of Mark
>Sent: Tuesday, June 06, 2006 2:23 PM
>Subject: [Histonet] overnight HIER
>Hi everyone.  In a meeting this morning someone mentionned that they had
>been toold to do an overnight HIER treatment.  They had never heard of
>this and wanted to know more. Is anyone doing this?  Is it simply
>heating the slides at say 60C for 12-18 hours in a water bath or is
>there more to it-what buffer, what temp??  Any information would be
>greatly appreciated.
>Mark Elliott
>Vancouver BC
>Histonet mailing list
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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