Mark,

In one of the workshops I attended in Toronto ( I wish I could remember
the name of the presenter), they mentioned doing this but not at 60
degrees C but a lower temp. like 37 or 45.  Hopefully this person will
see your inquiry and respond.  I believe they did this for finicky
antigens and tissues that were prone to come off the slides.

Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark
Elliott
Sent: Tuesday, June 06, 2006 2:23 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] overnight HIER


Hi everyone.  In a meeting this morning someone mentionned that they had
been toold to do an overnight HIER treatment.  They had never heard of
this and wanted to know more. Is anyone doing this?  Is it simply
heating the slides at say 60C for 12-18 hours in a water bath or is
there more to it-what buffer, what temp??  Any information would be
greatly appreciated.
Thanks
Mark Elliott
Vancouver BC


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