Hi Dorothy!

We routinely use this kit for our Esterases, and it works well for us.  The one thing you should tell your researcher, is that this kit is not specific to just neutrophils.  It is specific to any cell in the granulocytic lineage, so your basophils, and eosinphils will also stain.

We have had some research material stain negatively (this was mouse in our case) so I wonder if this kit is geared to human enzymatic activity specifically.  I would try calling Sigma and see it this is the case.

Otherwise, the insert states that there are a few things that can cause a false negative, although if your control works, I would say that it is something to do with the tissue itself (or the fixation). I don't think that expired formalin would do it as we get samples from Cameroon on a regular basis, and the formalin in them is definitely not great, and they work fine.

This may not help much, but I would try Sigma first and see if the kit has been designed for non-human tissue!

Best of Luck!

Kathy Johnston
Tech II Special Stains
Anatomic Pathology
Calgary Laboratory Services
#9 - 3535 Research Road NW
Calgary, AB.
403-770-3572


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Webb,
Dorothy L
Sent: June 9, 2006 6:58 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] neutrophils


We are staining some research tissue (rabbitt?) for neutrophils using
the chloroacetate esterase kit from Sigma with an inflamed appendix for
a control.  The control turned out beautiful, but, nothing is showing up
in the research tissue.  We did not  gross in the research tissue, only
processed it according to our routine processing schedule.  According to
the "fellow" who is doing this project, the formalin was expired on the
research tissue.  Does anyone have any ideas on this case??  I am at a
loss and the pathologist helping the "fellow" to read these tissues out
is wanting me to come up with a solution, of course!!  So, HELP and
thanks, as always!!
________________________________________
This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited.
 
If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you.


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet