RE: [Histonet] Trichrome staining and fibrin

From:"Marshall Terry Dr, Consultant Histopathologist"


You know full well that MSB required initial staining in mercury. I know that zinc is an adequate replacement.
Attempting to get a consistent good red colour with formalin fixed material is futile, as is post-fixation. 
At times it works and at times not.

IMO, Masson is useless for fibrin.

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire

-----Original Message-----
From: Bryan Hewlett []
Sent: 13 June 2006 15:52
To: Timo Väisänen;
Subject: Re: [Histonet] Trichrome staining and fibrin


Try the Lendrum MSB trichrome variant, it was designed to demonstrate 
I will send the method via a separate e-mail.


----- Original Message ----- 
From: "Timo Väisänen" 
Sent: Tuesday, June 13, 2006 10:33 AM
Subject: [Histonet] Trichrome staining and fibrin

> Hello all,
> I have struggled with getting Masson Trichrome (Goldner) staining to work 
> with
> kidney biopsies. The problem is that our pathologist does not get positive
> fibrin staining to glomeruli in diseased kidneys. Those areas of the 
> glomeruli
> that should contain fibrin, at least according to the pathologist, are 
> green
> (Lichtgrun) and not red, as they should be, whatever I do. I have tried to
> enhance the staining with Bouin's fixative (+56C) pretreatment but it did 
> not
> change the overall situation. However, the red stain was more intense in 
> skin
> samples containing fibrin (formalin fixed). I have also tried another 
> Trichrome
> staining with Crocein Scarlet 7B without any luck. In our lab kidney 
> biopsies
> are routinely fixed with alcoholic Bouin's. As far as I know it should be
> compatible with the Masson protocol we use. Any ideas? Any good protocols 
> that
> I could try?
> Thank's in advance,
> Timo
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