RE: [Histonet] Re:Sirius Red Protocol mouse pancreas
From: | "JEFF TATUM ZELIADT" |
______________________________________________________________
From: Jamie E Erickson
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re:Sirius Red Protocol mouse pancreas
Date: Thu, 15 Jun 2006 13:48:11 -0400
>Susan,
> We also do Sirius red staining but of mouse
lungs, we use
>a polarizing light to quantitative collagen deposition. We have
not see
>and appreciable increase in red staining over time. Maximum
staining
>intensity is reach after 90 minutes in the paper by L.C.U.
Junqueira
>Histochemical Journal 1979, 11,447-445. Check the histonet
achieves that's
>where I got most of my information.
> As far as the staining I would run an old sample
to
>determine if that stains differently then it had in the past, if
it does
>then it's a staining issue if not it's the tissue, it could be an
>experimental issue. Our investigators usually give us control
samples
>untreated Lung or we can ask for muscle or kidney for a positive
control
>I'd look into the tissue being the issue if you haven't already
done that.
>
>Hope that helps.
>
>Jamie
>
>_______________________________
>Jamie Erickson
>Sr. Research Associate
>Department: DSMP
>Abbott Bioresearch Center
>100 Research Drive
>Worcester, MA 01605-4341
>508-688-3134
>FAX: 508-793-4895
>e-mail: jamie.erickson@abbott.com
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