RE: [Histonet] Glucose Oxidase blocking for peroxidase, glucose source

From:"JEFF TATUM ZELIADT"


   remove my email from you damn lists fuck head
       ______________________________________________________________

     From:  Gayle Callis 
     To:  Histonet@lists.utsouthwestern.edu
     Subject:  [Histonet]   Glucose  Oxidase  blocking  for  peroxidase,
     glucose source
     Date:  Thu, 15 Jun 2006 11:34:51 -0600
     >To all using Glucose oxidase endogenous peroxidase blocking, be
     >aware that Sigma has discontinued the beta-D(+) glucose used for
     >this  blocking.  Some  have  tried another glucose as a substitute
     and
     >the peroxidase was not blocked.
     >
     >Since  I  tout  this  highly  useful peroxidase blocking method, I
     found
     >a source of the glucose
     >
     >ICN Cat#100953 beta-D Glucose.  this contain up to 10% of
     >alpha-isomer, although Sigma's contained only 4%.
     >
     >Hopefully  this  will  not  be discontinued as Sigma has done.  If
     there
     >are any doubts, call Sigma Technical Services and ask them for a
     >source of this glucose.
     >
     >Never thought there would be so much difference in a glucose
     >molecule
     >
     >
     >
     >
     >
     >
     >
     >Gayle Callis
     >MT,HT,HTL(ASCP)
     >Research Histopathology Supervisor
     >Veterinary Molecular Biology
     >Montana State University - Bozeman
     >PO Box 173610
     >Bozeman MT 59717-3610
     >406 994-6367
     >406 994-4303 (FAX)
     >
     >
     >
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