RE: [Histonet] Anti roll plate on IEC plus cryotome, need help
I have not used an IEC for some time but there were always problems with
the anti roll plate.
We ended up cutting glass ones of our own (from a 3 x 2" glass slide),
adding a thin strip of sellotape along each side to provide clearance
for the section and then spraying the surface with a Teflon spray to
prevent sections sticking. These plates were significantly better than
the plastic anti roll plates.
We never had much luck getting good serial sections of skin using the
brush technique but as Gayle says whatever works for you.
[mailto:email@example.com] On Behalf Of Gayle
Sent: Monday, June 05, 2006 4:39 PM
To: Ze Lu; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Anti roll plate on IEC plus cryotome, need help
The antiroll plate for this old IEC was probably developed for use with
profile steel knives.
I suggest you take the plate off and learn to do frozen sections using a
sable brush, or some type of brush that works best for you. A very nice
tutorial on how to use brush technic is found at
Talk to a technical services person at Triangle Biomedical Systems (?)
(TBS) about a different or workable antiroll plate or before you spend
money on a device that will be useless. TBS has a website and they sell
Personally, we prefer no antiroll plate and do brush technic for 99% of
cryostat work - it is faster, no readjustment of plates, etc.
At 02:02 PM 6/5/2006, you wrote:
>Hi, histonet friends,
>We have an old IEC plus cryotome. We changed to a disposable blade
>According to the IEC plus mannual, we adjust the anti roll plate.
>we tried couple sectioning, the section always curved and get folded. I
>used different crytome, for all of them, there is only small angle (or
>space) between blade holder and anti roll plate where secion go
>that section can not be folded after leave the block. However for IEC
>plus, it really has big angle according to their description. I think
>someone know what I am talking about if he used this model. Can someone
>share his experience on this? Also, we are going to change the anti
>plate, can someone kindly let us know which product will fit to this
>model. We appreciate very much.
>Ze Lu, Ph.D.,
>Optimum Therapeutics, LLC
>Histonet mailing list
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-4303 (FAX)
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