RE: Curious about this reply Re: [Histonet] RE: Disposal of Ventana DAB waste
I agree with Gayle. I don't think it's wise to put in the drain. Our
water department will not let ANY amount of DAB go down the drain no
matter how small the quantity.
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
800 Marshall Slot 820
Little Rock, AR 72202
visit us on the web at: www.archildrens.org
[mailto:firstname.lastname@example.org] On Behalf Of Gayle
Sent: Monday, June 12, 2006 12:57 PM
To: Emma JONES; Histonet@lists.utsouthwestern.edu
Subject: Curious about this reply Re: [Histonet] RE: Disposal of Ventana
Is this what Ventana advises? Labs should check with their local waste
water treatment plant before TREATING anything or putting "diluted" DAB
down the drain. If laboratories are generating this kind of waste on a
weekly basis, this adds up over time. In general, waste water plants do
like this and EPA does monitor water around here. Montana has discharge
rules (maybe for larger industries) but our city does not want
household cleaners, solvents, fertilizers, pesticides, oil, etc, flushed
down the drains and warn residents frequently about this in their water
Wouldn't it be better to collect and have it hauled away for proper
chemical disposal than add even "minute" amounts of a potential
to our water supply. We use very little DAB in our lab, but all
chromogens AEC, DAB, permanent red, etc, (very low volume usage) are
collected for chemical waste pickup and proper disposal.
An interesting sidelight, tested water wells in parts of Montana now
traces of sunscreen chemical, medicines, herbicides, nitrates and
nitrites. So much for the "pristine" environment and pure water out in
the Wild Wild West /Rocky Mountain region. Drink beer and brush you
with it too, it may be safer when you visit some Montana dude ranch!
Gayle Callis HTL, HT, MT(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717
At 11:19 AM 6/12/2006, you wrote:
>A specific answer to your question, regarding Ventana not DAKO waste.
>volume of DAB in the waste is very small and diluted considerably by
>other solvent free solutions.
>It can be thrown down the sink, washed down with plenty of water.
>However there is a technique for deactivation of DAB which is
>to any form of DAB waste.
>Prepare a 2 mol/litre H2SO4 solution, and a 0.2 mol/litre KMnO4
> - Add 50 ml of each solution to the Ventana DAB waste to be
> - Let the 3 solutions react overnight
> - Discard the solution into a regular sink, and wash down with
> plenty of water.
>Hope this is of use,
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