[Histonet] whole eyes

From:"Patsy Ruegg"

Dear Eye People,
It has been about 25 years since I processed eyes.  I just got some fresh
whole rabbit eyes that I want to fix and process into GMA.  They came in a
media with antibiotic in it one wet ice.  I assume that I put them whole
into cold formalin to maintain the shape, then at some point I will need to
extract the fluid inside without collapsing so that I can process into GMA.
I am going to use graded changes of the GMA as my dehydrating agent avoiding
alcohol (this will be necessary for the final product which will contain
hydrogel implant which does not survive solvent processing, thus the choice
of GMA embedding).  I seem to remember using a needle and syringe to extract
some fluid and then replace it with the GMA in stages????
Thank you so much for your advise,
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
wk email pruegg@ihctech.net
web site www.ihctech.net  

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