[Histonet] fixing sponge larvae for in situs
I have a colleague who wants to do in situ hybridizations on sponge
larvae (yes, sponges are animals and they have larval forms!). She
tried fixing some in 4% paraformaldehyde in sea water, and the larvae
exploded. She also tried Carnoy's fix, and 4% PFA in Millonig's
phosphate buffer, and the same thing happened.
The only thing I could think of to suggest was dropping to 1 or 2%
PFA in sea water. I've seen references to using Bouins for light
microscopy or osmium tetroxide for TEM, but I don't think either of
these approaches would be good for in situs.
Any other ideas?
Gary P. Radice firstname.lastname@example.org
Department of Biology 804-289-8107 (voice)
University of Richmond 804-289-8233 (FAX)
Richmond VA 23173 http://www.richmond.edu/~gradice
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