[Histonet] buffer rinses

From:"louise renton"

Hi all,

How long and/or vigorous should buffer rinses between immunohisto
steps be(ffpe 4um sections)? I am rinsing 5mins with agitation in PBS
+0.05% Tween. Is this too long? I am concerned that I am washing away
signal yet if I use PBS alone there is overwhelming background and
general muckiness

Louise Renton
Bone Research Unit
University of the Witwatersrand
South Africa
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