Hi all,

How long and/or vigorous should buffer rinses between immunohisto
steps be(ffpe 4um sections)? I am rinsing 5mins with agitation in PBS
+0.05% Tween. Is this too long? I am concerned that I am washing away
signal yet if I use PBS alone there is overwhelming background and
general muckiness

-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.

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