Susan,
                We also do Sirius red staining but of mouse lungs, we use 
a polarizing light to quantitative collagen deposition. We have not see 
and appreciable increase in red staining over time. Maximum staining 
intensity is reach after 90 minutes in the paper by L.C.U. Junqueira 
Histochemical Journal 1979, 11,447-445. Check the histonet achieves that's 
where I got most of my information. 
                 As far as the staining I would run an old sample to 
determine if that stains differently then it had in the past, if it does 
then it's a staining issue if not it's the tissue, it could be an 
experimental issue. Our investigators usually give us control samples 
untreated Lung or we can ask for muscle or kidney for a positive control 
I'd look into the tissue being the issue if you haven't already done that.

Hope that helps.

Jamie

_______________________________
Jamie Erickson
Sr. Research Associate 
Department: DSMP
Abbott Bioresearch Center
100 Research Drive
Worcester, MA 01605-4341
508-688-3134
FAX: 508-793-4895
e-mail: jamie.erickson@abbott.com
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