[Histonet] RE: Histonet Digest, Vol 31, Issue 17

From:"Featherstone, Annette"

We have our DAB waste disposed of by Safety Kleen.
Annette Featherstone

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
histonet-request@lists.utsouthwestern.edu
Sent: Monday, June 12, 2006 13:03
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 31, Issue 17


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Today's Topics:

   1. Re: Freezing small pieces of skeletal muscle tissue
      (Katri Tuomala)
   2. RE:Vectastain Elite kits (Till, Renee)
   3. RE: DAB disposal (Tom McNemar)
   4. Tissue control for CD56 (Castillos, Luminita)
   5. RE: DAB disposal (Anne Van Binsbergen)
   6. CAP question on humidity levels (Joe Nocito)
   7. Re: Tissue control for CD56 (Rene J Buesa)
   8. RE: Cryoprotection (Alan Bright)
   9. RE: CAP question on humidity levels (Dawson, Glen)
  10. RE: DAB disposal (Laurie Colbert)
  11. viability question (Fawn Jones)
  12. RE: CAP question on humidity levels (Andrea Grantham)
  13. Re: CAP question on humidity levels (Jennifer MacDonald)
  14. Minimizing shrinkage (Timothy Macatee)
  15. RE: Old Microtome.........knives
      (Edmondson David (RBV) NHS Christie Tr)
  16. Re: Minimizing shrinkage (Chris Pomajzl)
  17. why (Santana, Diane)
  18. Re: why (Jackie M O'Connor)
  19. RE: Minimizing shrinkage (Charles  Scouten)


----------------------------------------------------------------------

Message: 1
Date: Sun, 11 Jun 2006 19:29:07 -0400
From: "Katri Tuomala" 
Subject: Re: [Histonet] Freezing small pieces of skeletal muscle
	tissue
To: "Dearolf, Jennifer" ,
	
Message-ID: <006701c68dae$d5e7ec10$6a9a9618@Katri>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

Jenn,
I'm sure you'll get some good advice after the weekend from people who do=20
this all the time. My initial thought is, that your specimen should go from 
isopentane directly to -70C, not -20C, unless you cut it then. When you 
transfer the tissue to -70C, ice ctystal artifact will form, when tissue 
freezes slowly from -20 to -70.
Just a thought...

Katri
Katri Tuomala
Hamilton, Ontario, Canada

----- Original Message ----- 
From: "Dearolf, Jennifer" 
To: 
Sent: Friday, June 09, 2006 3:20 PM
Subject: [Histonet] Freezing small pieces of skeletal muscle tissue


Greetings, Histonetters,

I realize that freezing skeletal muscle tissue has been a topic many times 
before, but in going through the archives, I could not find anyone that was 
using the method that we are (and maybe that's the problem, since we are 
having issues with freezing artifact).  Thus, I am writing the list to see 
if anyone has any suggestions for how we might modify our methods to have=20
better results.

We collect our tissue fresh and mount it in 5% gum tragacanth on cork blocks 
(6mm thick).  To freeze the tissue, we put some isopentane in a frozen juice 
can (with the juice removed, of course!) and place the juice can in liquid 
nitrogen.  The liquid nitrogen is contained in a small, styrofoam cooler.=20
When the isopentane gets slushy, we freeze our samples for 60 seconds.  We 
then toss the frozen samples into a cryostat set at -21C.  Once the samples 
have warmed up and any liquid isopentane has evaporated, we wrap our samples 
in parafilm and store in cardboard boxes in a -70C freezer.  To cut, we move 
the boxes back into the cryostat and allow to wam up to      -21C for 
approximately an hour.  Then, we cut.

I used this method successfully with larger pieces of muscle, but we are now 
attempting to freeze some mouse and guinea pig muscles (diaphragm, scalenus, 
rectus thoracis, and rectus abdominus), and we are getting a ton of freezing 
artifact.  To try to prevent the artifact, we reduced the freezing time to 
20 seconds.  We also tried wrapping our samples in small pieces of liver.=20
Neither method seems to work consistently.  I am at my wits end.  Everytime 
I think we have the freezing artifact beaten, it comes back with a vengence.

I would appreciate any advice.  I will keep track of the temperature of the 
isopentane, since this seems to be an important variable to control 
(according to the discussions in the archives).  I would like to ask, 
however, should the temperature be -150 or -160C when you freeze the muscle? 
Thanks for your help!

Sincerely,
Jenn

Jennifer Dearolf, Ph.D.
Assistant Professor
Biology Department
Hendrix College
1600 Washington Avenue
Conway, AR 72032
(501) 450-4530 (office)
(501) 450-4547 (fax)
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 




------------------------------

Message: 2
Date: Mon, 12 Jun 2006 06:56:50 -0500
From: "Till, Renee" 
Subject: [Histonet] RE:Vectastain Elite kits
To: histonet@lists.utsouthwestern.edu
Message-ID:
	<11F927674DEBDC43B960809A7403C5D2012AC5F6@MAILPED.ad.uams.edu>
Content-Type: text/plain; charset=us-ascii

It's always worked fine for me. If I remember correctly, it does say
something in the package insert about not exchanging reagents from
different kits because they are optimized for that kit, but I've never
had any problems. I've even used the blocking serum and the secondary
from two different kits of the same species. 

 

Renee' Till, HT

Research Assistant

Arkansas Children's Nutrition Center

1212 Marshall St./N2021

Little Rock, AR 72002

Office (501)364-2785

Fax (501)364-3161

 

Message: 1

Date: Fri, 9 Jun 2006 12:29:00 -0500

From: jhaviland@mdanderson.org

Subject: [Histonet] Vectastain elite kits

To: histonet@lists.utsouthwestern.edu

Message-ID:

 


      

Content-Type: text/plain; charset=us-ascii

 

Dear Histonetters:

I am hoping someone has an answer  :  )

 

I had a crazy question run through my brain.  Is my thinking cap flawed
today...I am using a Vectastain Elite kit that is rabbit and one that is
mouse.  Is the ABC reagent the same in each kit and could be used
interchangeable  for either kit ( then I could save on making only one)
or is it animal specific?

Thanks

 

 

Joie

 

 


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------------------------------

Message: 3
Date: Mon, 12 Jun 2006 08:58:47 -0400
From: "Tom McNemar" 
Subject: RE: [Histonet] DAB disposal
To: "Joe Nocito" ,
	
Message-ID:
	<51D5D78FBEDAEA4FBCCD9A9D44211DC528F3C9@lmhsmail.lmhealth.org>
Content-Type: text/plain;	charset="iso-8859-1"

I have the Dako unit.  It separates the hazardous/non-hazardous waste.  The DAB goes into a 20L carboy that is then hauled off-site for disposal.


Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar@lmhealth.org
www.LMHealth.org



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe
Nocito
Sent: Friday, June 09, 2006 7:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] DAB disposal


with the risk of sounding stupid (which happens to me often) how are people disposing their DAB contaminated Ventana waste? I mean, there is so little actual DAB present. 
    I refuse to answer any questions that might incriminate me. I know what you are thinking.
    I have a new safety office who just happens to be certified in hazmat (yes, for those who know me, it's Hector). Hector keeps bugging me about it.
    For those who don't know us, Hector and I are joined at the hip. Last year in Ft. Lauderdale, everyone knew something was wrong because they saw Hector without me. No, we are not homosexuals, not that it matters. Hector was my histology instructor and we are really, really close friends.
    Oh d _ _ m, I'm sure I just offended somebody. I'm leaving now before I say something else wrong. It must be Friday. Why do I do this to myself? 
    Have a safe weekend y'all.

Joe Nocito BS, HT(ASCP)QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 4
Date: Mon, 12 Jun 2006 09:12:17 -0400
From: "Castillos, Luminita" 
Subject: [Histonet] Tissue control for CD56
To: 
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

Good morning everyone, 

I am working with human endometrial FFPE tissue and I am doing IHC for
NK using CD56 monoclonal antibody. I would like to ask which will be a
good positive tissue control and a negative tissue control  for CD56?.
Also, if there is a vendor from where I can buy these tissue slides?.
Thank you so much.

Sincerely, Luminita

Luminita Castillos, Ph.D.
Research Scientist
Cogenics(r), A Division of Clinical Data(r)
Comprehensive Pharmacogenomics & Molecular Services(tm)
108 Alexader Dr.
RTP, NC 27709
Tel:   919-425-2967
www.cogenics.com




------------------------------

Message: 5
Date: Mon, 12 Jun 2006 17:24:03 +0400
From: "Anne Van Binsbergen" 
Subject: RE: [Histonet] DAB disposal
To: "Tom McNemar" , "Joe Nocito"
	,	
Message-ID:
	
Content-Type: text/plain;	charset="US-ASCII"

Hi Tom
Today we became proud owners of a brand new Dako unit and I had that
exact thought in mind - what to do with a 20 litre bottle of
dab-contaminated waste....every couple of weeks - here in the UAE we
have little choice as there are no federal laws and no off-site waste
companies to cart waste off even for a fee. 
I am stuck until someone in authority writes the book of rules. 
I just know that one of you will go 'gasp!shock!horror'! and insist that
I be pro-active - I am - some of you know me to be 'tenacious' - this is
correct - but even that has not helped me here. Advice has been offered,
best practice quoted, OH&S quoted, internet sites accessed, printed,
handed over in report form, promises are made by those in authority and
then broken.
We stockpile safely off site and wait 
This is true for ALL toxic waste in this laboratory, as well as the rest
of the labs, together with mercury filled blood pressure cuffs, some
broken, 'expired' rat poison, old mercury thermometers, 'unknown'
unlabelled chemicals from shut down labs...some scary stuff
But - I digress - back to the DAB....and how to manage the growing
volume of dab waste.....
Any suggestions.....???
Anyone.....???

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom
McNemar
Sent: Monday, June 12, 2006 4:59 PM
To: Joe Nocito; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] DAB disposal

I have the Dako unit.  It separates the hazardous/non-hazardous waste.
The DAB goes into a 20L carboy that is then hauled off-site for
disposal.


Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar@lmhealth.org
www.LMHealth.org



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe
Nocito
Sent: Friday, June 09, 2006 7:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] DAB disposal


with the risk of sounding stupid (which happens to me often) how are
people disposing their DAB contaminated Ventana waste? I mean, there is
so little actual DAB present. 
    I refuse to answer any questions that might incriminate me. I know
what you are thinking.
    I have a new safety office who just happens to be certified in
hazmat (yes, for those who know me, it's Hector). Hector keeps bugging
me about it.
    For those who don't know us, Hector and I are joined at the hip.
Last year in Ft. Lauderdale, everyone knew something was wrong because
they saw Hector without me. No, we are not homosexuals, not that it
matters. Hector was my histology instructor and we are really, really
close friends.
    Oh d _ _ m, I'm sure I just offended somebody. I'm leaving now
before I say something else wrong. It must be Friday. Why do I do this
to myself? 
    Have a safe weekend y'all.

Joe Nocito BS, HT(ASCP)QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 6
Date: Mon, 12 Jun 2006 08:57:32 -0500
From: "Joe Nocito" 
Subject: [Histonet] CAP question on humidity levels
To: "Histonetters" 
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

            Okay, I can't stay quiet any longer. The new general checklist
question asks if the temperature and humidity levels are monitored. Okay,
but they don't state a range or what to do if the temp & humidity are out of
range. Has any one come up with an idea? And people wonder why I have a
problem with CAP.

Happy Monday. 

 

Joe Nocito, BS, HT(ASCP) QIHC

Histology Manager

Pathology Reference Lab

San Antonio, TX



------------------------------

Message: 7
Date: Mon, 12 Jun 2006 07:15:33 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] Tissue control for CD56
To: "Castillos, Luminita" ,
	histonet@lists.utsouthwestern.edu
Message-ID: <20060612141533.17828.qmail@web61216.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Luminita:
  For CD56 I used "brain" or "central nervous system" in general as a positive control.I never used any specific "negative" tissue, I just ran the negative slide without the primary Ab.
  The CD56 I used was from Novocastra with HIER at pH6
  Hope this will help you.
  Ren J.

"Castillos, Luminita"  wrote:
  Good morning everyone, 

I am working with human endometrial FFPE tissue and I am doing IHC for
NK using CD56 monoclonal antibody. I would like to ask which will be a
good positive tissue control and a negative tissue control for CD56?.
Also, if there is a vendor from where I can buy these tissue slides?.
Thank you so much.

Sincerely, Luminita

Luminita Castillos, Ph.D.
Research Scientist
Cogenics(r), A Division of Clinical Data(r)
Comprehensive Pharmacogenomics & Molecular Services(tm)
108 Alexader Dr.
RTP, NC 27709
Tel: 919-425-2967
www.cogenics.com


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




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------------------------------

Message: 8
Date: Mon, 12 Jun 2006 15:28:27 +0100
From: "Alan Bright" 
Subject: RE: [Histonet] Cryoprotection
To: "zwiegers" ,
	
Message-ID:
	
Content-Type: text/plain;	charset="iso-8859-1"

Dear Pierre,

Brain should be sectioned at -8 to -12C  and if possible have the knife & anti-roll plate around -20C. You are way too cold @ -30C Liver & Kidney should be no lower than -18C lower and you will just get dust=2E

I hope this helps if any more info is required please get back to me. Happy sectioning III

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abright@brightinstruments.com
Web Site: www.brightinstruments.com
Skype User ID: dazzle0


-----Original Message-----
From: zwiegers [mailto:zwiegers@interchange.ubc.ca] 
Sent: 10 June 2006 01:22
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryoprotection


Good day all,

Recently, we have sacrificed and perfused a batch of mice, and due to inattentiveness, some tissues (brain, spinal cord, liver, and kidney) were protected in 3% sucrose (instead of 30%). These were frozen and stored at -80 C for over a week. As soon as we started slicing the brain(s), we noticed that the tissue was shattering. At first we tried to reduce the temperature by keeping the tissue at - 30 C overnight. The tissue was still shattering when we tried to slice it. Now, since we used expired OCT, we assumed that this could have caused the problem. We then tried to use new OCT on a batch of mice that were not frozen yet (using 3% sucrose)and the results were the same.

Fortunately, we have not lost all of our tissue and they will just be transferred to a 30% sucrose solution. Now, the question is, what can we do with the frozen tissue? Is it at all possible to thaw the OCT  and transfer the organs to a 30% sucrose soltution with minimal damage to tissue integrity? 

Thank you so much in advance

Sara Sarband
Pierre Zwiegers


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Histonet mailing list
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------------------------------

Message: 9
Date: Mon, 12 Jun 2006 10:23:53 -0500
From: "Dawson, Glen" 
Subject: RE: [Histonet] CAP question on humidity levels
To: "Joe Nocito" ,	"Histonetters"
	
Message-ID:
	
Content-Type: text/plain;	charset="iso-8859-1"

Joe,

I posted a while ago on this same topic.  It's just another regulation that some CAP shut-in came up with while hanging out in a dark room trying to justify his/her job.  CAP just can't handle not coming up with at least a couple more crazy things to tack onto that CAP checklist every year.  Next year I hear we'll need to note barometric pressure, solar wind levels & sunspot activity.  

Since they don't offer a range, I would suggest just writing your readings down in a tablet so that you can say you have them.

Have Fun,

Glen Dawson  BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI  

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe
Nocito
Sent: Monday, June 12, 2006 7:58 AM
To: Histonetters
Subject: [Histonet] CAP question on humidity levels


            Okay, I can't stay quiet any longer. The new general checklist
question asks if the temperature and humidity levels are monitored. Okay,
but they don't state a range or what to do if the temp & humidity are out of
range. Has any one come up with an idea? And people wonder why I have a
problem with CAP.

Happy Monday. 

 

Joe Nocito, BS, HT(ASCP) QIHC

Histology Manager

Pathology Reference Lab

San Antonio, TX

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 10
Date: Mon, 12 Jun 2006 08:28:57 -0700
From: "Laurie Colbert" 
Subject: RE: [Histonet] DAB disposal
To: "Joe Nocito" ,	"Histonet (E-mail)"
	
Message-ID:
	<0BE6ADFAE4E7E04496BF21ABD346628005653BC4@EXCHANGE1.huntingtonhospital=2Ecom>
	
Content-Type: text/plain;	charset="iso-8859-1"


We have our licensed hazardous waste hauler take it.  I know there's not much DAB in the waste, but there's also the oily liquid coverslip that I don't want to pour down the drain.

Laurie Colbert

-----Original Message-----
From: Joe Nocito [mailto:jnocito@satx.rr.com]
Sent: Friday, June 09, 2006 4:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] DAB disposal


with the risk of sounding stupid (which happens to me often) how are people disposing their DAB contaminated Ventana waste? I mean, there is so little actual DAB present. 
    I refuse to answer any questions that might incriminate me. I know what you are thinking.
    I have a new safety office who just happens to be certified in hazmat (yes, for those who know me, it's Hector). Hector keeps bugging me about it.
    For those who don't know us, Hector and I are joined at the hip. Last year in Ft. Lauderdale, everyone knew something was wrong because they saw Hector without me. No, we are not homosexuals, not that it matters. Hector was my histology instructor and we are really, really close friends.
    Oh d _ _ m, I'm sure I just offended somebody. I'm leaving now before I say something else wrong. It must be Friday. Why do I do this to myself? 
    Have a safe weekend y'all.

Joe Nocito BS, HT(ASCP)QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 11
Date: Mon, 12 Jun 2006 11:32:57 -0400
From: Fawn Jones 
Subject: [Histonet] viability question
To: histonet@lists.utsouthwestern.edu
Message-ID: <448D8929.3000809@cs.cmu.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Dear histonetters,

I am looking for some information for a friend.  She wants to find out 
if her tissues (aortas) are live or not.  Does anyone have any 
experience performing viability stains on tissue samples?  She tried the 
Molecular Probes Live/Dead kit but found it insufficient due to 
permeabilty issues. She also suggested MTT, but we're not sure how well 
that stain works with tissues.  Any help would be greatly appreciated.

Thank you
Fawn Jones HT(ASCP)  



------------------------------

Message: 12
Date: Mon, 12 Jun 2006 08:40:00 -0700
From: Andrea Grantham 
Subject: RE: [Histonet] CAP question on humidity levels
To: "Dawson, Glen" ,	"Joe Nocito"
	,	"Histonetters"
	
Message-ID:
	<4.3.2.7.2.20060612083605.00ce3e98@algranth.inbox.email.arizona.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed

I remember many years back that the lab where I worked in TX had to get a=20
barometer in the lab as one of the outcomes of a CAP inspection and check=20
the humidity - this wasn't in Histology but in the Chemistry lab. Maybe 
that section of the CAP checklist has the answer to your question. Or - 
maybe you could ask someone in chemistry and save yourself the work of 
trying to find the answer and only look it up if you have to.
Andi



At 10:23 AM 6/12/2006 -0500, Dawson, Glen wrote:
>Joe,
>
>I posted a while ago on this same topic.  It's just another regulation 
>that some CAP shut-in came up with while hanging out in a dark room trying 
>to justify his/her job.  CAP just can't handle not coming up with at least 
>a couple more crazy things to tack onto that CAP checklist every 
>year.  Next year I hear we'll need to note barometric pressure, solar wind 
>levels & sunspot activity.
>
>Since they don't offer a range, I would suggest just writing your readings 
>down in a tablet so that you can say you have them.
>
>Have Fun,
>
>Glen Dawson  BS, HT & QIHC (ASCP)
>IHC Manager
>Milwaukee, WI
>
>-----Original Message-----
>From: histonet-bounces@lists.utsouthwestern.edu
>[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe
>Nocito
>Sent: Monday, June 12, 2006 7:58 AM
>To: Histonetters
>Subject: [Histonet] CAP question on humidity levels
>
>
>             Okay, I can't stay quiet any longer. The new general checklist
>question asks if the temperature and humidity levels are monitored. Okay,
>but they don't state a range or what to do if the temp & humidity are out of
>range. Has any one come up with an idea? And people wonder why I have a
>problem with CAP.
>
>Happy Monday.
>
>
>
>Joe Nocito, BS, HT(ASCP) QIHC
>
>Histology Manager
>
>Pathology Reference Lab
>
>San Antonio, TX
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
: (office:  AHSC 4212)          P.O. Box 245044                     :
: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
: (FAX:  520-626-2097)          (email:  algranth@u.arizona.edu)       :
:...................................................................:
           http://www.cba.arizona.edu/histology-lab.html




------------------------------

Message: 13
Date: Mon, 12 Jun 2006 08:58:56 -0700
From: Jennifer MacDonald 
Subject: Re: [Histonet] CAP question on humidity levels
To: "Joe Nocito" 
Cc: Histonetters ,
	histonet-bounces@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain; charset="US-ASCII"

Blood bank room temperatures are critical for correct testing.  This is on 
the general checklist, so it applies to the lab as a whole.






"Joe Nocito"  
Sent by: histonet-bounces@lists.utsouthwestern.edu
06/12/2006 06:57 AM

To
"Histonetters" 
cc

Subject
[Histonet] CAP question on humidity levels






            Okay, I can't stay quiet any longer. The new general checklist
question asks if the temperature and humidity levels are monitored. Okay,
but they don't state a range or what to do if the temp & humidity are out=20
of
range. Has any one come up with an idea? And people wonder why I have a
problem with CAP.

Happy Monday. 

 

Joe Nocito, BS, HT(ASCP) QIHC

Histology Manager

Pathology Reference Lab

San Antonio, TX

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 14
Date: Mon, 12 Jun 2006 11:50:06 -0400
From: Timothy Macatee 
Subject: [Histonet] Minimizing shrinkage
To: histonet@lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; charset=US-ASCII

I have someone who wants to measure cardiac vessel diameter.  Does anyone
have an idea of the best process to avoid shrinkage of the tissue?  Notice,
how I made no reference to any situation comedy television shows.

Tim  
-- 
Tim Macatee
Research Histology Core
New York University School of Medicine
550 First Ave.
Department of Pathology.
Medical Science Building - Room 521
New York, N.Y. 10016
(212) 263-3888





------------------------------

Message: 15
Date: Mon, 12 Jun 2006 17:23:39 +0100
From: "Edmondson David \(RBV\) NHS Christie Tr"
	
Subject: RE: [Histonet] Old Microtome.........knives
To: "Luck, Greg D." ,
	
Message-ID:
	<3C83687E8F6AE04792E361ABE2D385B8418129@cht-mail2-2k.xchristie.nhs.uk>
Content-Type: text/plain;	charset="us-ascii"

In the context of limited availability of servicing, I wonder at
Cambridge rockers,  they were not so flash but the worst servicing was a
new piece of twine and a spring.
The only rocker I know of, left over, came from a cryostat set up and
has a limited cutting stroke. 
But.....
If anyone wants a collection of Heiffer knives and various grades of
Alumina then please to ask.  They have not rusted away in spite of
twenty years and more in sheds. 
Also have a couple of regular stones and a rather long one.    What is
the smooth creamy coloured sharpening stone called, is it from somewhere
like Kansas?   And "Paul springs", that control the cam that engages in
the ratchet advance.
  
Dave
Manchester UK
  

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Luck,
Greg D.
Sent: 09 June 2006 22:44
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Old Microtome

Hello All,
 
A group of physicians and technicians are going on a mission trip to a
country in Africa in September.  They approached me about acquiring an
old unused microtome for them to take with them as there is no access to
histology services where they are going.  They have all the other
equipment they need for histology services.  If anyone has a microtome
(functional not fancy) they would be willing to donate to this
worthwhile mission I would be more than happy to pay for and facilitate
the shipping of the microtome to them.  
 
Thanks, Greg
 
Greg Luck
Anatomic Path Spvr
Deaconess Med Cntr
800 W. 5th Ave
Spokane, WA 99204
Offc 509.473.7077
Fax 509.473.7133
luckg@empirehealth.org
www.deaconessmc.org  
 
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------------------------------

Message: 16
Date: Mon, 12 Jun 2006 11:38:38 -0500
From: "Chris Pomajzl" 
Subject: Re: [Histonet] Minimizing shrinkage
To: "HISTONET" 
Message-ID: <008801c68e3e$a8394580$26fca8c0@CSP>
Content-Type: text/plain;	charset="iso-8859-1"

Don't put them in the pool. (sorry, had to say it)

We used to infuse vessels with a plastic polymer. Perhaps if you do this,
you could measure the diameter of the plastic assuming it would not shrink.

Also, what type of animal are we talking about?

----- Original Message ----- 
From: "Timothy Macatee" 
To: 
Sent: Monday, June 12, 2006 10:50 AM
Subject: [Histonet] Minimizing shrinkage


> I have someone who wants to measure cardiac vessel diameter.  Does anyone
> have an idea of the best process to avoid shrinkage of the tissue?
Notice,
> how I made no reference to any situation comedy television shows.
>
> Tim
> -- 
> Tim Macatee
> Research Histology Core
> New York University School of Medicine
> 550 First Ave.
> Department of Pathology.
> Medical Science Building - Room 521
> New York, N.Y. 10016
> (212) 263-3888
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 17
Date: Mon, 12 Jun 2006 12:40:25 -0400
From: "Santana, Diane" 
Subject: [Histonet] why
To: "'histonet@lists.utsouthwestern.edu'"
	
Message-ID: <4C96AA62BADFD81180CB0002A5AD67FB07113A3E@MAILPMA>
Content-Type: text/plain;	charset="iso-8859-1"

Hi 
I am looking for some ideas to help with a problem I am still having with
processing my GI bx's. What would cause my tissue to be raw? Not all, not
some, only 1 or 2 pieces. 
Example if I have a case with 4 bx's, maybe only 1 is raw, the other 3 are
beautiful. All my solutions test at the right % and temperatures are fine=2E 
Question again, why does 1 piece of tissue come out raw, when the other
pieces are fine?
thanks in advance,
Diane Santana
PMA
Haverhill, Mass.



------------------------------

Message: 18
Date: Mon, 12 Jun 2006 11:50:24 -0500
From: "Jackie M O'Connor" 
Subject: Re: [Histonet] why
To: "Santana, Diane" 
Cc: "'histonet@lists.utsouthwestern.edu'"
	,
	histonet-bounces@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain; charset="US-ASCII"

By raw I assume you mean "underprocesssed" (which isn't even a word) 
Anyway - do you use biopsy pads for processing?

Jackie O'



"Santana, Diane"  
Sent by: histonet-bounces@lists.utsouthwestern.edu
06/12/2006 11:40 AM

To
"'histonet@lists.utsouthwestern.edu'" 
cc

Subject
[Histonet] why






Hi 
I am looking for some ideas to help with a problem I am still having with
processing my GI bx's. What would cause my tissue to be raw? Not all, not
some, only 1 or 2 pieces. 
Example if I have a case with 4 bx's, maybe only 1 is raw, the other 3 are
beautiful. All my solutions test at the right % and temperatures are fine=2E 

Question again, why does 1 piece of tissue come out raw, when the other
pieces are fine?
thanks in advance,
Diane Santana
PMA
Haverhill, Mass.

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------------------------------

Message: 19
Date: Mon, 12 Jun 2006 11:56:24 -0500
From: "Charles  Scouten" 
Subject: RE: [Histonet] Minimizing shrinkage
To: "Chris Pomajzl" ,	"HISTONET"
	
Message-ID:
	<5784D843593D874C93E9BADCB87342AB013070CD@tpiserver03.Coretech-holdings.com>
	
Content-Type: text/plain;	charset="us-ascii"

 See the article in Microscopy Today.


Cordially,
Charles W.  Scouten, Ph.D. 
myNeuroLab.com 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300 x 342
FAX  314 522 0377 
cwscouten@myneurolab.com 
http://www.myneurolab.com 
 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris
Pomajzl
Sent: Monday, June 12, 2006 11:39 AM
To: HISTONET
Subject: Re: [Histonet] Minimizing shrinkage

Don't put them in the pool. (sorry, had to say it)

We used to infuse vessels with a plastic polymer. Perhaps if you do
this, you could measure the diameter of the plastic assuming it would
not shrink.

Also, what type of animal are we talking about?

----- Original Message -----
From: "Timothy Macatee" 
To: 
Sent: Monday, June 12, 2006 10:50 AM
Subject: [Histonet] Minimizing shrinkage


> I have someone who wants to measure cardiac vessel diameter.  Does
anyone
> have an idea of the best process to avoid shrinkage of the tissue?
Notice,
> how I made no reference to any situation comedy television shows.
>
> Tim
> -- 
> Tim Macatee
> Research Histology Core
> New York University School of Medicine
> 550 First Ave.
> Department of Pathology.
> Medical Science Building - Room 521
> New York, N.Y. 10016
> (212) 263-3888
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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Histonet@lists.utsouthwestern.edu
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------------------------------

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