[Histonet] RE: Histonet Digest, Vol 31, Issue 5
Dear Tiang Yee,
You can coat your slides using the following method.
Slide Coating with 3 - aminopropyltriethoxysilane (APES; Sigma A-3648)
1. Soak glass slides in acetone containing 2% APES for 1 to 5 mins;
2. Wash the slides in acetone or distilled water
3. Dry the slides at 42 °C.
All the best,
Langxing Pan, M.D., Ph.D.
457 Mariposa Street
San Francisco, CA 94107
Date: Sun, 4 Jun 2006 15:47:07 +0000
From: "tiang yeepeng"
Subject: Re: [Histonet] section adhesion on slides
Content-Type: text/plain; charset="iso-8859-1"
I coat my slides with poly-L-lysine but it seemed that I lost many cells
during the washing steps in IHC. I use a centrifuge my cells onto the slides
using the cytospin. Any idea or comment, Geoff?
TIANG YEE PENG
Department of Medical Microbiology,
Faculty of Medicine,
University of Malaya.
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