Dear Mark,

   I can echo Rebecca here: overnight HIER works fine with citrate pH6.0.
   Indeed,  Tris-EDTA pH9.0 is a bit aggressive to the tissue morphology.
   We  found overnight HIER extremely helpful with fatty tissues that are
   prone for floating.

   I  would  like  to  add  here  that one should not expect wonders from
   overnight  HIER:  the staining intensity for most antibodies is nearly
   the  same  as  with  "normal" HIER (15 min 100C). Of course we have an
   exception  here  (so  far):  CD3. That one stained much stronger after
   overnight HIER than with "normal" HIER.

   Lots of success!

   Chris van der Loos, PhD
   Dept. of Pathology
   Academic Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands

   

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