[Histonet] RE: HIER
I can echo Rebecca here: overnight HIER works fine with citrate pH6.0.
Indeed, Tris-EDTA pH9.0 is a bit aggressive to the tissue morphology.
We found overnight HIER extremely helpful with fatty tissues that are
prone for floating.
I would like to add here that one should not expect wonders from
overnight HIER: the staining intensity for most antibodies is nearly
the same as with "normal" HIER (15 min 100C). Of course we have an
exception here (so far): CD3. That one stained much stronger after
overnight HIER than with "normal" HIER.
Lots of success!
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
NL-1105 AZ Amsterdam
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