[Histonet] RE: Computer software to decrease background

From:"C.M. van der Loos"

   Dear all,

   There  is  software  (+  hardware) indeed to separate autofluorescence
   from  real  fluorescence. Spectral imaging is the way to unmix signals
   based  on  their  spectral  characteristics.  Autofluorescence  has  a
   slightly  different  spectral  characteristic than "real" fluorescence
   and can  therefore  be  nicely  separated  from each other. At the CRI
   website  ([1]http://www.cri-inc.com/products/nuance.asp) you should be
   able  to  find  some good examples of it. Sorry folks I work with that
   great  system  now  for  a  couple  of months for both brightfield and
   fluorescence, but I never considered it as being fraud.......

   NB: you will see it isn't "fraud" in NSH workshop #2: "Immunoenzymatic
   multiple  staining methods - A practical overview and possibilities of
   multi-color  imaging  by  spectral analysis" by Chris van der Loos and
   Richard Levenson. (Saturday September 9th, whole day)

   Chris van der Loos, PhD
   Dept. of Pathology
   Academic Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands

   Date: Thu, 08 Jun 2006 09:27:30 -0600
   From: Gayle Callis 
   Subject: Re: [Histonet] Computer software to decrease background
   staining in Immunoflouresence staining
   To: Geoff McAuliffe ,
   Geoff is absolutely correct!!!
   Gayle CAllis
   At 07:24 AM 6/8/2006, you wrote:
   >If  you  improve the quality of your immunostaining you won't need to
   do it
   >with  at  computer.  Removing/reducing   background  with  a computer
   >could   be   considered   fraud.   Most   journals  can  detect  such
   manipulations and
   >will reject your work on that basis alone.
   >sohail ejaz wrote:
   >>Hi every body
   >>   Is  there  any  one who can recommend me any Computer software to
   >> backgroun! d stainin


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