[Histonet] RE: Computer software to decrease background
Dear all,
There is software (+ hardware) indeed to separate autofluorescence
from real fluorescence. Spectral imaging is the way to unmix signals
based on their spectral characteristics. Autofluorescence has a
slightly different spectral characteristic than "real" fluorescence
and can therefore be nicely separated from each other. At the CRI
website ([1]http://www.cri-inc.com/products/nuance.asp) you should be
able to find some good examples of it. Sorry folks I work with that
great system now for a couple of months for both brightfield and
fluorescence, but I never considered it as being fraud.......
NB: you will see it isn't "fraud" in NSH workshop #2: "Immunoenzymatic
multiple staining methods - A practical overview and possibilities of
multi-color imaging by spectral analysis" by Chris van der Loos and
Richard Levenson. (Saturday September 9th, whole day)
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands
Date: Thu, 08 Jun 2006 09:27:30 -0600
From: Gayle Callis
Subject: Re: [Histonet] Computer software to decrease background
staining in Immunoflouresence staining
To: Geoff McAuliffe ,
Histonet@lists.utsouthwestern.edu,
[2]Histonet@lists.utsouthwestern.edu
Geoff is absolutely correct!!!
Gayle CAllis
At 07:24 AM 6/8/2006, you wrote:
>If you improve the quality of your immunostaining you won't need to
do it
>with at computer. Removing/reducing background with a computer
program
>could be considered fraud. Most journals can detect such
manipulations and
>will reject your work on that basis alone.
>
>Geoff
>
>sohail ejaz wrote:
>
>>Hi every body
>>
>> Is there any one who can recommend me any Computer software to
decrease
>> backgroun! d stainin
References
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1. http://www.cri-inc.com/products/nuance.asp
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