[Histonet] Help with Lung architecture

From:Alejandro Ortiz Stern


I am currently searching for a protocol to maintain Lung structure
intact while avoiding intra tracheal instillation of liquids since I am
very interested in cells that lie in the outer-most layers of the lung
(cells that would normally become detached upon lung lavage) Or if such 
cells remain attached during paraformaldehyde instillation or OCT 
instillation that would also be very valuable information (I have no idea).

If anyone has any suggestions, or knows how to fix/cut air inflated
lungs (for example if the trachea is closed before the opening of the
thoracic cavity or so) I would be extremely grateful for the advise.

The idea is to use these sections for confocal microscopy.

Thank You All in advance.

Alejandro Ortiz Stern M.D. Ph.D.,
Postdoctoral Fellow
Institut National de la Santé et de la Recherche Médicale, E0344
Université de Nice-Sophia-Antipolis
Institut de Pharmacologie Moléculaire et Cellulaire
660 Route des Lucioles - 06560 Valbonne - FRANCE
TEL: 33 (4) 93 95 77 80 - FAX: 33 (4) 93 95 77 08

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