[Histonet] Glucose Oxidase blocking for peroxidase, glucose source
To all using Glucose oxidase endogenous peroxidase blocking, be aware that
Sigma has discontinued the beta-D(+) glucose used for this blocking. Some
have tried another glucose as a substitute and the peroxidase was not
blocked.
Since I tout this highly useful peroxidase blocking method, I found a
source of the glucose
ICN Cat#100953 beta-D Glucose. this contain up to 10% of alpha-isomer,
although Sigma's contained only 4%.
Hopefully this will not be discontinued as Sigma has done. If there are
any doubts, call Sigma Technical Services and ask them for a source of this
glucose.
Never thought there would be so much difference in a glucose molecule
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)
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