Dear Histonetters,

I think I solved my problem I had with the edge artifact staining with
BK virus antibody. (BK virus large T antigen).
Instead of using Protease to unmask the antigen I changed to heat
induced epitope retrieval with Trilogy (Cell Marque)  and I also had to
dilute the antibody much further with this retrieval and so far I have
not seen the edge artifact again but I am doing some more today and hope
they are good as well.

Elaine Dooley
Shands Teaching Hospital
352-265-0111 ext 72117

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