Dear Histonetters,

In the last couple of weeks we have been getting nuclear staining on
the edges of most of our kidney biopsies when we stain them for BKV. 
Our negative control is negative. (both the negative and the BKV slides
are treated with a protease digestion and are blocked for endogenous
biotin).  The control tissue we have is a large piece of autopsy kidney
positive for BKV.  It does not have nuclear staining on the edges.  In
the past we never seen nuclear staining on the edges of our needle
biopsies.  The processing area says they have not changed anything in
the processing of kidney needle biopsies.  We use Chemicons BKV large T
antigen antibody we have tried many different  dilutions but so far we
are still seeing the artifact.  Has anyone out there had this problem?

Elaine Dooley
Shands Teaching Hospital
325-265-0111 ext. 72117



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