Who has the experiences on Anti-Glutamate Decarboxylase 65 & 67 (GAD 65/67) experiments?
  We use Anti-Glutamate Decarboxylase 65 & 67 (Chemcon, Cat. #: AB1511) as primary antibody, Biotin-SP-AffiniPure Goat Anti-Rabbit IgG (Jackson, Cat. #: 111-065-144) as secondary antibody. The serum is from goat. We stain mouse sections. The problem is that the experiments worked perfect every time before. But the God is making jokes with me now: none of recent experiments works. Any suggestions are highly appreciated. 

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