[Histonet] A Call for Help from Melbourne/OZ
My name is Scott Sinclair, and I am a PhD student in the Department
of Genetics (@ The University of Melbourne/Australia) and am hoping
to get your advice on the following:
I have been using a fluorescent dye (Zinpyr) to try to image Zinc in
the roots of Arabidopsis seedlings/plant, using a confocal
microscope. I'm also interested in looking at paraffin sections of
roots, therefore wondering if there is a 'fixing' process that will
leave the intracellular localisation of Zinc undisturbed while
examining sections/Will routine fixation in 10% NBF or 70% ETOH be
fine? I know you have to fix sections before looking at protein or
DNA/RNA, and was wondering if it can be done for ions also?
Scott Sinclair (sent through Bruce)
DEPT. of ZOOLOGY
THE UNIVERSITY Of MELBOURNE. VICTORIA. AUSTRALIA 3010
Nobody Can Make You Feel Inferior Without YOUR Permission -
Q: What's a specimen?
A: An Italian astronaut.
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