Re: [Histonet] uneven haematoxylin/eosin staining

From:"Karen Percival"

Hi Diana,

It sounds like a processing problem problem to me.  You are not getting
good infiltration.  I would check the tissue processor for proper
functioning and change all reagents as well if the processor functions
look as if they are working properly.  Check all reagents on the
processor, too, for quality.  Start with fresh everything for the next
run.  I have found that it's usually  a reagent problem (usually
technician error) if everything has been looking great and then all of a
sudden something is wrong.  


Karen Percival
Research Scientist I
Wyeth Research
1 Burtt Road
Andover, MA 01810
888-577-1500 x 4058

>>> Diana McCaig  6/28/2005 1:19:18 PM >>>
We have been experiencing uneven staining recently.  Even when all
are cut on the same microtome and put in the same rack on an
their macroscopic appearance show incredible variations.  It is so
random we
can not identify the problem.  The Harris hemotoxylin is filtered
The tissue type does not make any difference.  We can have 4 prostate
and 2 will be good and 2 will show random staining intensities.  Recuts
show variation in a different area which makes me think it is the
and not the cutting.

Any suggestions would be truly appreciated.
Diana McCaig, MLT 

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