Re: [Histonet] uneven haematoxylin/eosin staining

From:Paul Bradbury

Sounds like a classical case of incomplete removal of wax before 
staining begins. Check the de-waxing reagents and times.

Paul Bradbury
Kamloops, BC

Diana McCaig wrote:

>We have been experiencing uneven staining recently.  Even when all sections
>are cut on the same microtome and put in the same rack on an autostainer,
>their macroscopic appearance show incredible variations.  It is so random we
>can not identify the problem.  The Harris hemotoxylin is filtered daily.
>The tissue type does not make any difference.  We can have 4 prostate slides
>and 2 will be good and 2 will show random staining intensities.  Recuts may
>show variation in a different area which makes me think it is the staining
>and not the cutting.
>Any suggestions would be truly appreciated.
>Diana McCaig, MLT 
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