Re: [Histonet] uneven haematoxylin/eosin staining
Sounds like a classical case of incomplete removal of wax before
staining begins. Check the de-waxing reagents and times.
Diana McCaig wrote:
>We have been experiencing uneven staining recently. Even when all sections
>are cut on the same microtome and put in the same rack on an autostainer,
>their macroscopic appearance show incredible variations. It is so random we
>can not identify the problem. The Harris hemotoxylin is filtered daily.
>The tissue type does not make any difference. We can have 4 prostate slides
>and 2 will be good and 2 will show random staining intensities. Recuts may
>show variation in a different area which makes me think it is the staining
>and not the cutting.
>Any suggestions would be truly appreciated.
>Diana McCaig, MLT
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