Re: [Histonet] elastic stain
One of the nicest, simplest stains for elastic fibres (large, small, and
tiny) is orcein. The fibres are deep purple and no differentiation steps
are involved. It's an ideal method for mass production. However, the
purple of the elastin does not contrast well with the red of the van
Gieson. Aldehyde fuchsin is also great for elastic fibres, does not
require differentiation, and is reliable. Its contrast with van Gieson
is not great though.
A number of years ago, Keith Gordon and I wrote up a method in
Bancroft's Theory and Practice of Histological Techniquers that used a
dye called Dahlia, one of the triphenylmethane dyes related to the basic
fuchsins. This method gave very good, reliable results, needed no
differentiation, and stained the elastic fibres a very dark blue (almost
black) that contrasted nicely with the van Gieson. The stain solution is
essentially a varaint of aldehyde fuchsin, but uses dahlia in place of
Kamloops, BC, Canada
Steven Coakley wrote:
>Good morning everyone,
>Is there any elastic stain thats reliable for batch staining, that still give the domonstration of fine/course of a good VVG? Also has anyone the potassium permanganate/oxalic acid prior to the elastic working solution to achieve better staining of elastic fibers? Any opinions on whether using sodium thiosulfate or 95% alcohol is better for removing the iodine?
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