Re: [Histonet] Wrinkles in cartilage sections

From:louise renton


try placing your slide briefly on a hot plate after picking the
section up from the waterbath before allowing it to drain. This used
to work on lungs with bronchus, so i gues it might do for you. The
only problem you might encounter is if the tissue is poorly processes
in which case the other stuff might "blow-up"

Hope this is some help

On 6/21/05, Kristen Broomall  wrote:
> I need some "O wise one" suggestions!
> I'm sectioning sheep trachea & the cartilage layer is coming out full of
> wrinkles. The epithelium layer is nice, flat & happy (it is, I asked it), as
> is the muscle. The tissue is FFPE and I'm cutting at 5-6 um. The sections
> look good in the ribbon, but as soon as they hit the water bath, the
> cartilage wrinkles up.
> I've tried:
> *ethanol in the water bath
> *ammonia in the water bath
> *tween in the water bath
> *soaking the paraffin blocks in ammonia water before cutting
> *increased water bath temperature
> Suggestions???
> Kristen Broomall, HT (ASCP)
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> Histonet mailing list

Louise Renton
Bone Research Unit
University of the Witwatersrand
South Africa
"....I know who I am. No-one else knows who I am. If I was a giraffe,
and someone said I was a snake, I'd think no, actually I'm a giraffe"
Richard Gere

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