Re: [Histonet] Oil Red O mountant


Hello Andi,

I have monting my fat slide with a monting medium specific for Oil Red O

Protocol is:

Apathy's Mouting Media

50 g sucrose PA
50 g Acacia (gum arabic) PA
150 ml Distilled Water
0.1 g Thymol
Mix Acacia and distilled water in a Becker. Place the Becker in a pan of
boiling water until Aacia is dissolved. Than mix sucrose until dissolved and
put on a Thymol. Refrigarete to remove air bubbles.

Carffull with bubbles.

MariaPaula Montiani Scandar
Department of Pathology
Labory of Neuropathology
School of Medicine of Ribeirão Preto
University of São Paulo

----- Original Message -----
From: "Anita Jennings" 
Sent: Wednesday, June 22, 2005 10:42 AM
Subject: [Histonet] Oil Red O mountant

> Andi
> I had the same problem. initially with my fat stain.....Poly Scientific
> K043 contains the glycerine jelly mounting medium specific for Oil Red
> coverslipping, havent had a problem since I started ordering this kit
> 800-645-5825
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