I also use the tab 250 her-2 from zymed, with proteinase, and get great 
results.
anita dudley
providence hosp. mobile alabama
>From: ddittus787@aol.com
>To: ddittus787@aol.com, eca9@georgetown.edu, 
>histonet@lists.utsouthwestern.edu
>Subject: Re: [Histonet] Her2 antibody
>Date: Thu, 23 Jun 2005 12:24:19 -0400
>
>As a side note zymed also has Tab 250 clone of cerb-2 that only need enzyme 
>and then incubation and gives awesome reuslts as well.                      
>      dana
>
>-----Original Message-----
>From: ddittus787@aol.com
>To: eca9@georgetown.edu; histonet@lists.utsouthwestern.edu
>Sent: Wed, 22 Jun 2005 16:29:03 -0400
>Subject: Re: [Histonet] Her2 antibody
>
>
>anti-her 2 cb11 clone works very well with citrate hier dilution(depending 
>on
>your method is 1:40-1:100)
>and a 30 minute incubation at 37 degrees.
>                                                                         
>dana
>
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>-----Original Message-----
>From: Eva C Andersson 
>To: histonet@lists.utsouthwestern.edu
>Sent: Wed, 22 Jun 2005 14:30:14 -0400
>Subject: [Histonet] Her2 antibody
>
>
>Hello,
>I just ordered mouse anti-Her2 cat.no. 18-7107 from Zymed. There is however 
>no
>indication given as to which dilution to use. I want to use it for
>Immunohistochemistry on paraffin embedded sections. It also doesn't say 
>anything
>about antigen retrieval.
>Is anyone using this antibody and if so could you tell me what you do?
>Thanks,
>Eva Andersson
>Georgetown University
>
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