Re: [Histonet] GFAP

From:Geoff McAuliffe

So what does the staining look like? Smooth muscle cells, fibroblasts, 
endothelial cells or ?? Exactly where in the artery (intima, media, 
adventitia)?
Did you run a negative control, one without primary antibody? If not you 
could be picking up endogenous peroxidase activity.
Not all arteries are the same so variations may not mean much.

Geoff

Bruijntjes, J.P. wrote:

>Hi all
>
>Is anyone of you familiar with the staining of GFAP on muscular structures of blood vessels and bronchi/bronchioli in na´ve rats. The staining which appeared in my sections was not slight or weak, but really strong.
>
>As a 'positive control' I used slides of the brain (rat tissue). In these brain slides I found a rather big artery and that vessel was 100% negative? 
>
>The antibody I have used is a polyclonal antibody from Dako (code Z0334).
>
>Does anyone of you know why those vessels in the lungs are positive for GFAP?
>
>Joost Bruijntjes
>TNO Quality of Life
>Zeist
>Holland
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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