RE: [Histonet] (no subject)
Dear Mr Bruijntjes,
I am assuming your brain tissue and microtome chamber are at -12ºC not 12ºc, If you lowered only the chamber temperature to -20ºC to lower the knife and anti-roll plate it will return your smile.
Best Regards
Alan Bright
Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England
Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abright@brightinstruments.com
Web Site: www.brightinstruments.com
-----Original Message-----
From: Bruijntjes, J.P. [mailto:bruyntjes@voeding.tno.nl]
Sent: 22 June 2005 11:02
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
Hi all
I was wondering if anyone of you is used to cut non-fixed cryoslides of brain tissue of about 30-40 micron thick. I've tried a few peaces on a rather high temperature (approximately 12ºC), but it did not make me smile?
J.P. Bruijntjes
TNO Quality of Life
Zeist
Holland
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