RE: [Histonet] fixation question

From:"Bonner, Janet"

 In practice, do we use NBF after Bouin's fixation?, after alcohol/formalin?
after BNF?  She would probably be better off doing what is done in practice
and not on Mars for a realistic study on comparisons of the fixatives.

-----Original Message-----
Sent: 6/29/2005 3:19 PM
Subject: Re: [Histonet] fixation question

Once tissue has been properly fixed ,NBF should have no huge effect on
H&E, sometimes over-fixing (2 or more fixatives) can affect special
stains, IHC,IN-situ. If I were a student and had 3 fixatives I would
wait to get past the formalin step to be able to purely judge fixation
issues of each independent fixative. You know what we learn and what
happens in the real world is always different.
-----Original Message-----
From: Bryan Llewellyn 
To: Histonet 
Sent: Wed, 29 Jun 2005 11:22:41 -0700
Subject: Re: [Histonet] fixation question

If she is comparing the effects of three different fixatives, then she
should go into the post-fixative part of the schedule. Otherwise she is
comparing double fixation of two of them. That is another, seperate,
Bryan Llewellyn 
----- Original Message ----- From: "Scholz, Stephen J."
Sent: Wednesday, June 29, 2005 10:59 AM 
Subject: [Histonet] fixation question 
> Hello all; 
> I have a question about some fixatives. We have a student whom is
using > our lab in conjunction with an on-line course to prepare for the
exam. > She was asked to fix tissue in three different fixatives and
compare the > results on H&E. She has chosen Bouins, formalin/alcohol
and 10% BNF. We > use the BNF as routine in our lab. My question is
after she has fixed the > tissue in the other two fixatives can she put
the specimen back into the > 10% BNF for routine processing or does she
need to wait until the > processor goes passed its formalin changes in
order to add her specimens? > I have had no experience with using a
fixative other than 10% BNF as a > primary so I don't know what to tell
> Thank you for your help, 
> Steve Scholz 
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