Try a liver from primary biliary cirrhosis or even a cirrhotic liver. The
copper will be seen around the edge of the hepatic nodules.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: Malam Jacqueline [mailto:Jacqueline.Malam@rli.mbht.nhs.uk] 
Sent: Thursday, 16 June 2005 6:42 PM
To: Histonet submissions (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] Stain and pos control for copper


I wonder if anyone with experience of copper stains can help me. We very
rarely get asked to demonstrate copper and I know of only one positive case
of Wilson's disease in 15 years which we were able to use as a pos control.
We have just had another request. There was barely any control tissue left
and both the test and control was neg. when stained with rhodanine. Two
questions - is rhodanine the best stain, and what can we use for a pos.
control?
All ideas gratefully received!
Jacqui Malam
Lancaster Infirmary
England



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