RE: [Histonet] Silly question about deparaffinization
Wow... Those times all seem huge! We've always just used 2 x 2 min
Xylene, 2 x 1 min 100% EtOH, 1 x 1 min 90% EtOH, 1 x 1 min 70% EtOH then
to water. No problems so long as the solutions are changed when they
start to get dirty.
From: Sebree Linda A. [mailto:email@example.com]
Sent: Thursday, 30 June 2005 2:14 a.m.
To: Eva C Andersson; firstname.lastname@example.org
Subject: RE: [Histonet] Silly question about deparaffinization
We deparaffinize in 3 changes of xylene, 2 changes of 100% ETOH, 1 95%
ETOH and 1 70% ETOH, all for 3 minutes each. We never skimp on the
xylene times but have been known to shorten the ETOH times if we
manually agitate the slides and are in a real hurry.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
600 Highland Ave.
Madison, WI 53792
[mailto:email@example.com] On Behalf Of Eva C
Sent: Wednesday, June 29, 2005 8:57 AM
Subject: [Histonet] Silly question about deparaffinization
I am probably asking a very silly question but I recently spoke to
someone about deparaffinization and hydration. I found that the times
that they use and the times that we use are very different. So my
question to you is this: What lenghts of time do you use for Xylenes,
100% alcohol, 95% alcohol and 70% alcohol?
It is about deparaffinising charged (+) slides. So nothing fancy. Just
curious. Maybe I can cut back some on the timed that we use which are:
30min Xylenes, 10min 100%, 10min 95% and 6min 70%. Thanks for your
responses. Eva Andersson Georgetown University
Histonet mailing list
Attention: The information contained in this message and/or attachments
from AgResearch Limited is intended only for the persons or entities
to which it is addressed and may contain confidential and/or privileged
material. Any review, retransmission, dissemination or other use of, or
taking of any action in reliance upon, this information by persons or
entities other than the intended recipients is prohibited by AgResearch
Limited. If you have received this message in error, please notify the
Histonet mailing list
<< Previous Message | Next Message >>