RE: [Histonet] Silly question about deparaffinization
Wow... Those times all seem huge! We've always just used 2 x 2 min
Xylene, 2 x 1 min 100% EtOH, 1 x 1 min 90% EtOH, 1 x 1 min 70% EtOH then
to water. No problems so long as the solutions are changed when they
start to get dirty.
From: Sebree Linda A. [mailto:firstname.lastname@example.org]
Sent: Thursday, 30 June 2005 2:14 a.m.
To: Eva C Andersson; email@example.com
Subject: RE: [Histonet] Silly question about deparaffinization
We deparaffinize in 3 changes of xylene, 2 changes of 100% ETOH, 1 95%
ETOH and 1 70% ETOH, all for 3 minutes each. We never skimp on the
xylene times but have been known to shorten the ETOH times if we
manually agitate the slides and are in a real hurry.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
600 Highland Ave.
Madison, WI 53792
[mailto:firstname.lastname@example.org] On Behalf Of Eva C
Sent: Wednesday, June 29, 2005 8:57 AM
Subject: [Histonet] Silly question about deparaffinization
I am probably asking a very silly question but I recently spoke to
someone about deparaffinization and hydration. I found that the times
that they use and the times that we use are very different. So my
question to you is this: What lenghts of time do you use for Xylenes,
100% alcohol, 95% alcohol and 70% alcohol?
It is about deparaffinising charged (+) slides. So nothing fancy. Just
curious. Maybe I can cut back some on the timed that we use which are:
30min Xylenes, 10min 100%, 10min 95% and 6min 70%. Thanks for your
responses. Eva Andersson Georgetown University
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