Wow... Those times all seem huge! We've always just used 2 x 2 min
Xylene, 2 x 1 min 100% EtOH, 1 x 1 min 90% EtOH, 1 x 1 min 70% EtOH then
to water. No problems so long as the solutions are changed when they
start to get dirty.

Euan McNaughton

-----Original Message-----
From: Sebree Linda A. [mailto:la.sebree@hosp.wisc.edu] 
Sent: Thursday, 30 June 2005 2:14 a.m.
To: Eva C Andersson; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Silly question about deparaffinization

We deparaffinize in 3 changes of xylene, 2 changes of 100% ETOH, 1 95%
ETOH and 1 70% ETOH, all for 3 minutes each.  We never skimp on the
xylene times but have been known to shorten the ETOH times if we
manually agitate the slides and are in a real hurry.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX:  (608)262-7174



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eva C
Andersson
Sent: Wednesday, June 29, 2005 8:57 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Silly question about deparaffinization


Good morning,
I am probably asking a very silly question but I recently spoke to 
someone about deparaffinization and hydration. I found that the times 
that they use and the times that we use are very different. So my 
question to you is this: What lenghts of time do you use for Xylenes, 
100% alcohol, 95% alcohol and 70% alcohol?
It is about deparaffinising charged (+) slides. So nothing fancy. Just
curious. Maybe I can cut back some on the timed that we use which are:
30min Xylenes, 10min 100%, 10min 95% and 6min 70%. Thanks for your
responses. Eva Andersson Georgetown University

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