I am pleased to report back to those who are interested that the procedure I recommended for brain sectioning problems solved  Joost's problems.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abright@brightinstruments.com
Web Site: www.brightinstruments.com



-----Original Message-----
From: Bruijntjes, J.P. [mailto:bruyntjes@voeding.tno.nl] 
Sent: 23 June 2005 09:55
To: Alan Bright
Subject: RE: [Histonet] (no subject)


Hi Alan

You are right, my smile is back. Thanks for your remarks.

Joost Bruijntjes
TNO Quality of Life
Zeist
Holland

-----Original Message-----
From: Alan Bright [mailto:abright@brightinstruments.com] 
Sent: woensdag 22 juni 2005 12:35
To: Bruijntjes, J.P.; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject)

Dear Mr Bruijntjes, 

		I am assuming your brain tissue and microtome chamber are at  -12ºC not 12ºc, If you lowered only the chamber temperature to -20ºC to lower the knife and anti-roll plate it will return your smile.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abright@brightinstruments.com
Web Site: www.brightinstruments.com



-----Original Message-----
From: Bruijntjes, J.P. [mailto:bruyntjes@voeding.tno.nl] 
Sent: 22 June 2005 11:02
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)


Hi all

 

I was wondering if anyone of you is used to cut non-fixed cryoslides of brain tissue of about 30-40 micron thick. I've tried a few peaces on a rather high temperature (approximately 12ºC), but it did not make me smile? 

 

J.P. Bruijntjes

TNO Quality of Life

Zeist

Holland

 


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