RE: [Histonet] BCIP/NBT ready-to-use

From:"Edmondson David (RBV) NHS Christie Tr"

DAKO do complete reagent in one, and the BCIP/NBT/INT that we use, its all used within date so can not help with long term stability.   No ppt formed that we have seen

Christie Hosp
Manchester UK
-----Original Message-----
[]On Behalf Of Johnson,
Sent: 15 June 2005 23:22
To: Histonet
Subject: [Histonet] BCIP/NBT ready-to-use

We are currently using a substrate system which has two separate
components for BCIP and NBT, and then have to combine them just before
use.  I'm looking into alternate systems and have seen several vendors
who offer what appears to be ready-to-use substrate. Is anybody using
them and can you comment on stability with regard to expiration date,
length of use, and lack of problems after the slides have been stained
(I recall a recent thread regarding horrible overstaining after the
slides had been coverslipped).  Thanks muchly!

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110

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