[Histonet] preservation of the stratum corneum in skin cryosections
From: | "Birthe Schnegelsberg" |
Hello.
Does anybody have a method to keep the stratum corneum attached to the
epithelium using in vitro skin cryosections?
I am freezing in vitro skin without fixation in isopentane chilled in liquid
nitrogen. I don' t see any stratum corneum in my 5-7um HandE stained
samples.
Thanks, Birthe
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