[Histonet] post-fixing quick frozen tissues for IHC
Hello all,
I was wondering if anyone has had any problems with tissues that were
quick frozen before being being fixed and cryoprotected? We usually obtain
our tissues fresh, fix in some sort of paraformaldehyde solution (Zamboni's,
Lanas), cryoprotect in sucrose solution for around 1 week, and then quick
freeze. However, for our positive controls, we are only able to obtain
quick frozen tissues that haven't yet been fixed. I suppose that this might
affect the achitecture a bit, but does anyone have any knowledge of whether
immunohistochemistry might be affected? We are looking for nerve
innervation, and will be obtaining muscle and skin for these purposes....
-Phil
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