Hello all,

   I was wondering if anyone has had any problems with tissues that were 
quick frozen before being being fixed and cryoprotected?  We usually obtain 
our tissues fresh, fix in some sort of paraformaldehyde solution (Zamboni's, 
Lanas), cryoprotect in sucrose solution for around 1 week, and then quick 
freeze.  However, for our positive controls, we are only able to obtain 
quick frozen tissues that haven't yet been fixed.  I suppose that this might 
affect the achitecture a bit, but does anyone have any knowledge of whether 
immunohistochemistry might be affected?  We are looking for nerve 
innervation, and will be obtaining muscle and skin for these purposes....

-Phil



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