[Histonet] RE: Histonet Digest, Vol 19, Issue 25
I am sure the the Sturkey company who now makes disposables is still sharpening knivesmike riceholy cross hospFt lauderdale-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Ofhistonet-request@lists.utsouthwestern.eduSent: Friday, June 17, 2005 1:07 PMTo: histonet@lists.utsouthwestern.eduSubject: Histonet Digest, Vol 19, Issue 25Send Histonet mailing list submissions to histonet@lists.utsouthwestern.eduTo subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonetor, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.eduYou can reach the person managing the list at histonet-owner@lists.utsouthwestern.eduWhen replying, please edit your Subject line so it is more specificthan "Re: Contents of Histonet digest..."Today's Topics: 1. RE: formic acid decal (DiCarlo, Margaret) 2. RE: xylene substitutes (Shirley Powell) 3. Re: Species specific antibody marker (Jackie M O'Connor) 4. RE: knife sharpening (Due, Brice) 5. RE: Alizarin S mindbender question (Shirley Powell) 6. Histotechnician position available (Spoon, Victoria) 7. Romulin red (Paula Pierce) 8. Re: Romulin red (Jackie M O'Connor) 9. Rodent mast cell IHC (Thomas Crowell) 10. Knife Sharpeneing (HACKERLAB@aol.com) 11. elastic stain (Steven Coakley) 12. Red chromogens (Patti Loykasek) 13. RE: formic acid decal (Elizabeth Chlipala) 14. RE: elastic stain (Patsy Ruegg) 15. RE: Rodent mast cell IHC (Patsy Ruegg)----------------------------------------------------------------------Message: 1Date: Fri, 17 Jun 2005 09:54:51 -0400From: "DiCarlo, Margaret" Subject: RE: [Histonet] formic acid decalTo: 'Trisha Emry' , histo Message-ID: <139141F8BAF4A642A945ECC528511AF001F5ECE5@kalmb02.kaleidahealth.org>Content-Type: text/plain; charset="iso-8859-1"Trisha,I make my own 10% formic acid decal solution and use for large bone sampleswithout any damage. And I get nice H&E staining.Peggy DiCarlo HT (ASCP)Orthopedics Bone LabBuffalo General Hospital100 High St.Buffalo, NY 14203716-859-1293 -----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of TrishaEmrySent: Thursday, June 16, 2005 17:54To: histoSubject: [Histonet] formic acid decalI need to speed up the decal process on some large bone samples.What is the highest precentage of formic acid that I can use withoutdamaging the bones?I am using sodium formate with formic acid now.Thanks,TrishaSeattle_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonetCONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777.------------------------------Message: 2Date: Fri, 17 Jun 2005 10:04:51 -0400From: Shirley Powell Subject: RE: [Histonet] xylene substitutesTo: histology@gradymem.org, 'Histonet' Message-ID: <01LPKB3AZ07K8WXLUG@Macon2.Mercer.edu>Content-Type: text/plain; charset=us-asciiI have tried Formula 83 from CBG Biotech and it works quite well. Shirley -----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Ofhistology@gradymem.orgSent: Friday, June 17, 2005 6:56 AMTo: HistonetSubject: [Histonet] xylene substitutesDoes anyone currently use xylene substitues in their H&E staining series?If so, which one have you found to clear best? Did you try several to cometo that conclusion?We have tried several in the past and are currently using RA ClearRite.However, all of the sudden our pathologist thinks we can get betterclearing. (I think he has been looking at xylene cleared slides from areference lab and remembers how much better xyleneclears.)If anyone has had wonderful results with any brand of clearing please let usknow.Thanks,Angie Barnett, HTL(ASCP)Grady Memorial HospitalChickasha, OK_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet------------------------------Message: 3Date: Fri, 17 Jun 2005 09:08:17 -0500From: "Jackie M O'Connor" Subject: Re: [Histonet] Species specific antibody markerTo: Colin Nixon Cc: histonet@lists.utsouthwestern.eduMessage-ID: Content-Type: text/plain; charset="us-ascii"Affinity BioReagents, (ABR) has a Mouse MAB (vimentin) which is specific for Mouse, Human and Rat. (MA3-745). You can use this to distinguish murine from canine cells, theoretically. I've used a human mitochondria marker to distinguish human from murine - everything that stains is human - the ones that don't stain are murine. This should work the same, I would hope. I haven't found a murine mitochondria marker so far.JackieJacqueline M. O'Connor HT(ASCP) QIHCAssistant ScientistGPRD Cancer ResearchAbbott Laboratories, Abbott Park, ILJackie.OConnor@abbott.com"Colin Nixon" Sent by: histonet-bounces@lists.utsouthwestern.edu06/17/2005 08:28 AMPlease respond to Colin Nixon To: cc: Subject: [Histonet] Species specific antibody markerI have received FFPE mouse tissue that has been treated with canine MDCK cells. It has been established that there are carcinoma cells present in the mice tissue. What I am trying to establish is whether the tumour has originated from a canine or murine origin. Does anyone know of any immunohistochemical markers that would be species specific for this task?Thanks,Colin_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet------------------------------Message: 4Date: Fri, 17 Jun 2005 10:19:43 -0400From: "Due, Brice" Subject: RE: [Histonet] knife sharpeningTo: "Greer, Patricia" , "Histonet \(Histonet\)" Message-ID: <59C772E2D8EDF345AE1601F5B60B3CB50102705A@PHSXMB7.partners.org>Content-Type: text/plain; charset="iso-8859-1"You can also try C.L. Sturkey www.sturkey.com -- they are in PA.-brice"THE INFORMATION TRANSMITTED IN THIS EMAIL IS INTENDED ONLY FOR THE PERSON ORENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGEDMATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKINGOF ANY ACTION IN RELIANCE UPON, THIS INFORMATION BY PERSONS OR ENTITIES OTHERTHAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVE THIS EMAIL IN ERROR,PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER."HIPPA Policy, 2003, Brigham & Women's Hospital, Boston, MA-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Greer,PatriciaSent: Friday, June 17, 2005 6:46 AMTo: Histonet (Histonet)Subject: [Histonet] knife sharpeningDoes anyone know of a company that sharpens microtome steel knives? Acolleague here is have a problem with using disposable blades on hiscryostat. The steel knife works well, but now needs sharpening. Welong ago disposed of our knife sharpener and I seem to remember thatthere are companies that does microtome knife sharpening. Thanks, Pat GreerCenters for Disease Control and PreventionInfectious Disease Pathology Activity1600 Clifton RoadAtlanta, GA 30333_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet------------------------------Message: 5Date: Fri, 17 Jun 2005 10:23:19 -0400From: Shirley Powell Subject: RE: [Histonet] Alizarin S mindbender questionTo: 'histonet' Message-ID: <01LPKBQ7HYL88WXW7U@Macon2.Mercer.edu>Content-Type: text/plain; charset=iso-8859-1Julien, I have performed the Dawson's technique (page 549 in CFA Culling thirdedition) on human embryos years ago using Alizarin to stain bone. I am notsure if this is suitable for what you are doing but if you contact me I willbe happy to talk to you about what I encountered in the procedure. Youremail address did not show up in the message. My email address ispowell_sa@mercer.edu or you can call me.Shirley PowellMercer University School of MedicineMacon, GA 478-301-2374-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman,Barry RSent: Friday, June 17, 2005 8:46 AMTo: histonetSubject: RE: [Histonet] Alizarin S mindbender questionHi JulienGreetings from Houston where it is never cold. Come visit us sometime if youwant to escape from the north.I have not worked with fish apart from ingesting several; however I haveused an alizarin/ alcian blue technique several times to stain bone andcartilage for frogs and rodents.The technique that you use removes soft tissues and I am unclear as to whythis is necessary. The techniques that I have used have retained the softtissue and have made them permeable to clearing agents so that the detailsof bone and cartilage are very clear.My first thought on your technique is that the KOH is made up too stronglyby mistake, can easily happen or that there is still trypsin present andacting (although the usual pH range for trypsin is 7 to 9). Both wouldexplain the soft tissue maceration.I am not sure how long you use the trypsin or its concentration although itgenerally is fast acting and it is usually only active for about 3 hours.Trypsin can be sold with some gelatin in it as a stabilizing agent but notsure if this is the case here.The cloudiness may be a reaction between remnants of trypsin and thealizarin.Just some thoughts, not sure if these are correct or just BS.Good luckBarry-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JulienLambrey de SouzaSent: Wednesday, June 08, 2005 3:30 PMTo: histonet@lists.utsouthwestern.eduSubject: [Histonet] Alizarin S mindbender questionHello histonetters,In our lab we do routine staining of fish specimens by the Clear and staintechnique of Dingerkus and Uhler 1977, and some of the existingmodifications with very nice results.But in the last few days, this technique has turned sour....Something is happening to the fish when they are left in the Alizarin red Ssolution made up with 0.5% KOH. The solution has been made fresh severaltimes but gives the same result each time. The fish seem to contract(muscular?) so much that the caudal becomes deformed (S shaped), the finrays curl up and detach and the cranial elements also seem very fragile. Myfirst thought was that the KOH solution was too harsh, but then I realizedthat the same KOH (0,5%) is used with these fish in the bleaching solutionwithout harming the fish (apart from normal bleaching).The protocol is summarized as follows:1) dehydration in EtOH ---- the fish turn out fine2) stain in alcian blue solution (EtOH+acetic acid+alcain blue 8GN)---- fishturn out fine3) neutralization in borax ---- fish are still fine4) bleach in H2O2 solution (H2O2 3%+ KOH 0,5%) ---- fish still very nice5) trypsin digestion (borax+H2O+tryspin) ------ although trypsin has clearedthe specimen of its "meat" the fish is still very nice and fin rays areintact.6) Alizarin red S solution (KOH 0,5%+alizarin S to turn solution deeppurple) ----- almost on contact with the solution, the fish rinkles, rayscurl up and detach... the specimen becomes useless. (The solution used tocolor the bones red and the specimen was then cleared of excess red by KOHbath).I have tried changing borax and KOH solutions with no improvement. Could it be that Alizarin S has gone bad? Is this even possible? We have hadvery high temperatures in the lab lately (30 degrees celsius), could thishave influenced the solutions? I am puzzled. Also, we keep our trypsin inthe -20´C freezer. The freezer has had a problem and freezed to -40´C for aday. Could this have altered the trypsin in a way that specimen expositionto this trypsin is incompatible with a later exposition to alizarin? (I amout of good ideas, so I'm trying absurd ideas....).If anyone is willing to take a shot, I will accept all suggestions because Iam high and dry.Thanks for any help.Julien De souza,Evolutionary biology,UQAR, Quebec._______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet=------------------------------Message: 6Date: Fri, 17 Jun 2005 10:25:47 -0400From: "Spoon, Victoria" Subject: [Histonet] Histotechnician position availableTo: Message-ID: <052739589974CC44A7770DA22157C804099B5D@ex3.bassett.org>Content-Type: text/plain; charset="iso-8859-1"Bassett Healthcare in Cooperstown, New York has a part-time histotechnician position available immediately with the strong possibility of becoming full time within a year. Responsibilities include full range of histotechniques including embedding, sectioning, routine, special and immuno staining in a hospital based laboratory. If interested, contact:Victoria Spoon, Anatomic Pathology ManagerBassett HospitalCooperstown, NY 13326email: victoria.spoon@bassett.org(607)547-6357NOTICE OF CONFIDENTIALITYThis electronic message, including attachments, is for the sole use of thenamed recipient and may contain confidential or privileged information protected by New York State, and Federal regulations. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. If you are not the intended recipient or have received this communication in error please contact the sender or email.security@bassett.org and destroy all copies of the original message. Thank you.------------------------------Message: 7Date: Fri, 17 Jun 2005 07:50:54 -0700 (PDT)From: Paula Pierce Subject: [Histonet] Romulin redTo: histonet@lists.utsouthwestern.eduMessage-ID: <20050617145054.85393.qmail@web50301.mail.yahoo.com>Content-Type: text/plain; charset=iso-8859-1I thought Romulins looked like Vulcans, but were bad, hung out at the neutral zone and had the cloaking device. Maybe romulin red is like schiff's and is "cloaked" until used. ;)Paula Pierce, HTL(ASCP)HTExcalibur Pathology, Inc.630 N. BroadwayMoore, OK 73160405-759-3953contact@excaliburpathology.comwww.excaliburpathology.com------------------------------Message: 8Date: Fri, 17 Jun 2005 09:57:18 -0500From: "Jackie M O'Connor" Subject: Re: [Histonet] Romulin redTo: Paula Pierce Cc: histonet@lists.utsouthwestern.eduMessage-ID: Content-Type: text/plain; charset="us-ascii"Biocare is Star Trek oriented - kinda cool and fun - reminds me of doing business with Ben and Jerry's. (Hey, Hari - send me some ice cream). They actually have a "Decloaking Chamber" - their pressure cooker for HIER - as well as a Borg solution for HIER. Check out their website at www.Biocare.net if you haven't already.Nice people, too - I might add.I used the Romulin Red - and it worked just fine. I think, however, that a Methyl Green counterstain is best with it - kinda Christmas-sy.Jackie O'Paula Pierce Sent by: histonet-bounces@lists.utsouthwestern.edu06/17/2005 09:50 AM To: histonet@lists.utsouthwestern.edu cc: Subject: [Histonet] Romulin redI thought Romulins looked like Vulcans, but were bad, hung out at the neutral zone and had the cloaking device. Maybe romulin red is like schiff's and is "cloaked" until used. ;)Paula Pierce, HTL(ASCP)HTExcalibur Pathology, Inc.630 N. BroadwayMoore, OK 73160405-759-3953contact@excaliburpathology.comwww.excaliburpathology.com_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet------------------------------Message: 9Date: Fri, 17 Jun 2005 10:58:02 -0400From: Thomas Crowell Subject: [Histonet] Rodent mast cell IHCTo: histonet@lists.utsouthwestern.eduMessage-ID: Content-Type: text/plain; charset="US-ASCII"Can anyone tell me if DakoCytomations C-kit CD117 polyclonal antibody crosses with mouse FFPE mast cells?ThanksTom CrowellBiogenIdecCambridge, MA------------------------------Message: 10Date: Fri, 17 Jun 2005 10:59:14 EDTFrom: HACKERLAB@aol.comSubject: [Histonet] Knife SharpeneingTo: pwg1@cdc.gov, Histonet@pathology.swmed.eduMessage-ID: <1ee.3dec0215.2fe43f42@aol.com>Content-Type: text/plain; charset="US-ASCII" Hi Pat -I thought I'd pass another option your way - Hacker Instruments located in Winnsboro, South Carolina sharpens and reconditions knives as well. We also manufacture/distribute equipment and accessories used in Pathology/Histology - check out our web site, _www.hackerinstruments.com_ (http://www.hackerinstruments.com/) ; or give us a call; 803) 712-6100.Be SafeShawnelle Shawnelle PowellProcurement/PurchasingHacker Instruments803) 712-6100 ext 20------------------------------Message: 11Date: Fri, 17 Jun 2005 08:07:04 -0700 (PDT)From: Steven Coakley Subject: [Histonet] elastic stainTo: Histonet@lists.utsouthwestern.eduMessage-ID: <20050617150704.1180.qmail@web90206.mail.scd.yahoo.com>Content-Type: text/plain; charset=iso-8859-1Good morning everyone, Is there any elastic stain thats reliable for batch staining, that still give the domonstration of fine/course of a good VVG? Also has anyone the potassium permanganate/oxalic acid prior to the elastic working solution to achieve better staining of elastic fibers? Any opinions on whether using sodium thiosulfate or 95% alcohol is better for removing the iodine? Steve ---------------------------------Do you Yahoo!? Yahoo! Mail - Find what you need with new enhanced search. Learn more.------------------------------Message: 12Date: Fri, 17 Jun 2005 08:11:11 -0700From: Patti Loykasek Subject: [Histonet] Red chromogensTo: histonet Message-ID: Content-Type: text/plain; charset="US-ASCII"Just to add a quick note, I like the Dako permanent red chromogen. It is foruse with alkaline phosphatase detections. It is nice & bright, easy to use.Great for double labeling, too.Patti LoykasekPhenoPath LaboratoriesSeattle, WA------------------------------Message: 13Date: Fri, 17 Jun 2005 09:17:28 -0600From: "Elizabeth Chlipala" Subject: RE: [Histonet] formic acid decalTo: "'Trisha Emry'" , "'histo'" Message-ID: <001401c5734f$adf76750$a7d48a80@AMY>Content-Type: text/plain; charset="us-ascii"TrishaI have used 20% formic acid made up in distilled water on sheep tibiasand it worked just fine.LizElizabeth A. Chlipala, BS, HTL(ASCP)QIHCManagerPremier Laboratory, LLCP.O. Box 18592Boulder, Colorado 80308Office: (303) 735-5001Fax: (303) 735-3540liz@premierlab.comwww.premierlab.com Ship to Address:Premier LaboratoryUniversity of ColoradoMCDB, Room A3B40Boulder, Colorado 80309-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of TrishaEmrySent: Thursday, June 16, 2005 2:54 PMTo: histoSubject: [Histonet] formic acid decalI need to speed up the decal process on some large bone samples.What is the highest precentage of formic acid that I can use withoutdamaging the bones?I am using sodium formate with formic acid now.Thanks,TrishaSeattle_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet------------------------------Message: 14Date: Fri, 17 Jun 2005 10:28:46 -0600From: "Patsy Ruegg" Subject: RE: [Histonet] elastic stainTo: "'Steven Coakley'" , Message-ID: <200506171628.j5HGSgt1005594@chip.viawest.net>Content-Type: text/plain; charset="us-ascii"I use Movat's Pentachrome as a batch stain for elastic fibers. I get thekit from Polyscientific and reuse many of the reagents.Patsy -----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of StevenCoakleySent: Friday, June 17, 2005 8:07 AMTo: Histonet@lists.utsouthwestern.eduSubject: [Histonet] elastic stainGood morning everyone, Is there any elastic stain thats reliable for batch staining, that stillgive the domonstration of fine/course of a good VVG? Also has anyone thepotassium permanganate/oxalic acid prior to the elastic working solution toachieve better staining of elastic fibers? Any opinions on whether usingsodium thiosulfate or 95% alcohol is better for removing the iodine? Steve ---------------------------------Do you Yahoo!? Yahoo! Mail - Find what you need with new enhanced search. Learn more._______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet------------------------------Message: 15Date: Fri, 17 Jun 2005 10:29:34 -0600From: "Patsy Ruegg" Subject: RE: [Histonet] Rodent mast cell IHCTo: "'Thomas Crowell'" , Message-ID: <200506171629.j5HGTUt1005826@chip.viawest.net>Content-Type: text/plain; charset="us-ascii"Not in my experience.Patsy -----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ThomasCrowellSent: Friday, June 17, 2005 7:58 AMTo: histonet@lists.utsouthwestern.eduSubject: [Histonet] Rodent mast cell IHCCan anyone tell me if DakoCytomations C-kit CD117 polyclonal antibodycrosses with mouse FFPE mast cells?ThanksTom CrowellBiogenIdecCambridge, MA_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet------------------------------_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonetEnd of Histonet Digest, Vol 19, Issue 25****************************************----------------------
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