[Histonet] RE: Blocking serum
Dear Baowei Peng,
Since you have a goat anti-rabbit IgG as second step reagent you
should use normal goat serum for pre-blocking step. Otherwise use
Protein Block, serum-free (DakoCyto X0909).
Chris van der Loos, PhD
Dept. of Pathology
Academical Medical Center M2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands
phone: +31 20 5665631
fax: +31 20 6960389
Date: Wed, 29 Jun 2005 08:35:04 +0800 (BEIST)
From: Baowei Peng
Subject: [Histonet] Blocking serum
To: histonet@lists.utsouthwestern.edu
Message-ID: <20050629003504.7EDCD11104FD@sjtu.edu.cn>
Content-Type: text/plain; charset="gb2312"
Hello, all
I\'m doing IHC on mouse frozen sections. The first antibody in my hand
is Rabbit polyclonal antibody,the second antibody is Goat anti Rabbit
IgG(H+L). I\'m wondering what kind of serum I should use to block non
specific staining.
Thanks in advance!
Baowei Peng
1954 Huashan Road
Pharmacy School
Shanghai Jiaotong University
Shanghai, 200030
China
Ph:86-21-62932108
E-mail:pengbw@sjtu.edu.cn
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<< Previous Message | Next Message >>