[Histonet] Frozen sections for IF
From: | "Catherine Stanecki" |
Hi Histonetters,
So, I'm pretty new to histology and I have several questions about frozen
sections. I would really appreciate your help! First of all, how long are
frozen tissues good for after freezing in tissue tek OTC? Is it better to
store at -20 C or -80 C? Also, how long are sections on slides good for
before you have stained and how should you store them (i.e. in a slide box
in the freezer)? How far in advance can you cut your sections before
staining them?
Thanks so much for you help!
Cathy
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